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AAT Bioquest

XFD555 Anti-human CD116 Antibody *4H1, XFD555 Same Structure to Alexa Fluor™ 555*

The 4H1 monoclonal antibody binds to human CD116, a 70 - 85 kD glycoprotein commonly located on the surface of endothelial precursors and macrophages. CD116 is associated with a variety of biologically interesting macromolecules/ligands, for instance, CD131 and GM-CSF. CD116 is a relatively rare antibody target, with fewer than 100 publications in the last decade. Even still, CD116 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD555 (ex/em = 553/568 nm). XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 561 nm laser and 582/15 nm bandpass filter (for example, as in the BD FACSMelody™).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd116 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on December 17, 2024

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
1116015011160151
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Antibody properties

Other namesGM-CSFRα chain
Clone4H1
Hostmouse
IsotypeMouse IgG1 kappa
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.08

Correction Factor (280 nm)

0.08

Extinction coefficient (cm -1 M -1)

150000

Excitation (nm)

553

Emission (nm)

568

Quantum yield

0.11

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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