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Trypan Blue, sodium salt *CAS 72-57-1*

Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue. Live cells or tissues with intact cell membranes are not be colored. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue color under a microscope. Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells. It is also useful to observe hyphea of fungi and Stramenopiles.

Example protocol

AT A GLANCE

Important notes
It is important to store at room temperature.

It can be used within 6 months from the date of receipt.

Cat. #

                      Product Name

  Unit Size

 2450

      Trypan Blue, sodium salt *Cell culture tested*

  100 g

 2452

       Trypan Blue, sodium salt *UltraPure  grade* *Purified to eliminate fluorescent impurities*

  10 g

 2455

     Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*

  1 mL

 2456

     Trypan Red Plus™ *0.1 M aqueous solution*

  10 mL

 2457

     Trypan Red Plus™ *0.1 M aqueous solution*

  100 mL

 2465

    Trypan Purple™ *0.1 M aqueous solution*

  10 mL

 2466

    Trypan Purple™ *0.1 M aqueous solution*

  100 mL

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Trypan Blue, sodium salt *CAS 72-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM104.079 µL520.394 µL1.041 mL5.204 mL10.408 mL
5 mM20.816 µL104.079 µL208.158 µL1.041 mL2.082 mL
10 mM10.408 µL52.039 µL104.079 µL520.394 µL1.041 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

References

View all 106 references: Citation Explorer
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium
Authors: Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.
Journal: Graefes Arch Clin Exp Ophthalmol (2005): 917
Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells
Authors: Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.
Journal: Invest Ophthalmol Vis Sci (2005): 304
Page updated on October 28, 2024

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Catalog Number2450
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Physical properties

Molecular weight

960.81

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Room temperature (10-25 °C); Minimize light exposure
UNSPSC12352200

CAS

72-57-1