Name: trFluor™ Eu Acceptor XL665-Streptavidin Conjugate
Brand: AAT Bioquest
SKU: 1441
Price: 348 USD
Availability: InStock

trFluor™ Eu Acceptor XL665-Streptavidin Conjugate

trFluor™ Eu Acceptor XL665-Streptavidin Conjugate is a time-resolved FRET acceptor for developing sensitive TR-FRET assays when paired with europium-labeled probes.

TR-FRET Acceptor: XL665 acceptor dye conjugated to streptavidin for developing sensitive time-resolved FRET assays when paired with Eu donors
Ready-to-Use Format: Pre-conjugated streptavidin enables immediate binding to any biotinylated probe or biomolecule without additional labeling steps
Background Suppression: Long emission half-life allows time-gated detection that eliminates autofluorescence from biological samples and improves assay sensitivity
High Stability: Superior photostability and emission yield compared to traditional fluorophores ensures consistent, reliable assay performance

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1441	100 ug	Price

Overview

trFluor™ Eu Acceptor XL665-Streptavidin Conjugate is an excellent building block for developing TR-FRET assays. It is labeled with the trFluor™ Eu Acceptor XL665 that is an excellent replacement for the D2 acceptor. The acceptor is commonly used to label antibodies or antigens to prepare the bioconjugates that are used to pair with Eu-labeled probes for developing TR-FRET assays. trFluor™ Eu Acceptor XL665-Streptavidin Conjugate can be readily used with a biotinylated Eu luminescent probe such as antibodies. Eu probes enable time-resolved fluorometry (TRF) for the assays that require high sensitivity. They have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our TR Fluor™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. The trFluor™ Eu probes are excellent donors for developing TR-FRET assays by pairing with trFluor™ Eu Acceptor XL665-Streptavidin Conjugate. Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores lead to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences.

References

View all 50 references: Citation Explorer

Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology.

Authors:

Payne, N Connor and Ichikawa, Saki and Woo, Christina M and Mazitschek, Ralph

Journal:

STAR protocols (2024): 103129

Development of a high-throughput TR-FRET screening assay for a fast-cycling KRAS mutant.

Authors:

Larson, Jacob E and Hardy, P Brian and Schomburg, Noah K and Wang, Xiaodong and Kireev, Dmitri and Rossman, Kent L and Pearce, Kenneth H

Journal:

SLAS discovery : advancing life sciences R & D (2023): 39-47

Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα and PPARγ.

Authors:

Roth, Doris and Benz, Jörg and Grether, Uwe and Dietz, Michel

Journal:

Methods in molecular biology (Clifton, N.J.) (2023): 155-169

Deep Drug Discovery of Mac Domain of SARS-CoV-2 (WT) Spike Inhibitors: Using Experimental ACE2 Inhibition TR-FRET Assay, Screening, Molecular Dynamic Simulations and Free Energy Calculations.

Authors:

Iqbal, Saleem and Lin, Sheng-Xiang

Journal:

Bioengineering (Basel, Switzerland) (2023)

Development of a high-throughput TR-FRET screening assay for LAG-3/FGL1 interaction.

Authors:

Abdel-Rahman, Somaya A and Zhang, Longfei and Gabr, Moustafa T

Journal:

SLAS discovery : advancing life sciences R & D (2023): 188-192

Physical properties

Solvent

Water

Spectral properties

Excitation (nm)	652
Emission (nm)	659

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 25, 2025