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trFluor™ Eu Acceptor XL665
trFluor™ Eu Acceptor XL665 is an excellent replacement for the commonly used D2 acceptor in labeling antibodies or antigens for bioconjugates paired with europium (Eu)-labeled probes in TR-FRET assays. Eu probes enable time-resolved fluorometry (TRF) in assays requiring high sensitivity. They exhibit large Stokes Shifts and exceptionally long emission half-lives compared to traditional fluorophores such as Alexa Fluor or cyanine dyes. In comparison to other TRF compounds, our TR Fluor™ Eu probes offer high stability, high emission yield, and the ability to be linked to various biomolecules. These probes serve as excellent donors for developing TR-FRET assays in conjunction with trFluor™ Eu Acceptor XL665. Since many biological compounds found in cells, serum, or other biological fluids are naturally fluorescent, using conventional, prompt fluorophores can significantly limit assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. Utilizing long-lived fluorophores combined with time-resolved detection, which introduces a delay between excitation and emission detection, minimizes prompt fluorescence interferences.
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References
View all 50 references: Citation Explorer
Efficient Eu3+-Integrated UiO-66 Probe for Ratiometric Fluorescence Sensing of Styrene and Cyclohexanone.
Authors: Zhang, Zhijuan and Liu, Luping and Zhang, Teng and Tang, Hanxiao
Journal: ACS applied materials & interfaces (2023): 18982-18991
Authors: Zhang, Zhijuan and Liu, Luping and Zhang, Teng and Tang, Hanxiao
Journal: ACS applied materials & interfaces (2023): 18982-18991
Time-Resolved FRET-Based Immunosensor for the Ultrasensitive and Rapid Detection of Cd2.
Authors: Han, Zi and Pan, Yi and Chen, Shouhui and Wei, Xinlin
Journal: Journal of agricultural and food chemistry (2023): 11195-11203
Authors: Han, Zi and Pan, Yi and Chen, Shouhui and Wei, Xinlin
Journal: Journal of agricultural and food chemistry (2023): 11195-11203
Eu doped Ti3C2 quantum dots to form a ratiometric fluorescence platform for visual and quantitative point-of-care testing of tetracycline derivatives.
Authors: Fu, Chaojun and Ai, Fanrong and Huang, Jianzhen and Shi, Zhiying and Yan, Xiluan and Zheng, Xiangjuan
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 120956
Authors: Fu, Chaojun and Ai, Fanrong and Huang, Jianzhen and Shi, Zhiying and Yan, Xiluan and Zheng, Xiangjuan
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 120956
Comparative analysis of lanthanide excited state quenching by electronic energy and electron transfer processes.
Authors: Parker, David and Fradgley, Jack D and Delbianco, Martina and Starck, Matthieu and Walton, James W and Zwier, Jurriaan M
Journal: Faraday discussions (2022): 159-174
Authors: Parker, David and Fradgley, Jack D and Delbianco, Martina and Starck, Matthieu and Walton, James W and Zwier, Jurriaan M
Journal: Faraday discussions (2022): 159-174
Monitoring of pesticide amount in fruit and vegetables by a fluorescence-based sensor.
Authors: Rodrigues, Andreia Cm and Barbieri, Maria Vittoria and Febbraio, Ferdinando
Journal: EFSA journal. European Food Safety Authority (2022): e200419
Authors: Rodrigues, Andreia Cm and Barbieri, Maria Vittoria and Febbraio, Ferdinando
Journal: EFSA journal. European Food Safety Authority (2022): e200419
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