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TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*
Positively charged rhodamine dyes (such as rhodamine esters and rosamines) are selectively localized in mitochondria, thus they are widely used for labeling mitochondria of live cells. Like JC-1, TMRM and TMRE are widely used for measuring mitochondrial membrane potential besides their selective mitochondrial staining. These two particular rhodamine esters stain mitochondria orange in fluorescence. Their spectral properties are similar to those of TRITC, making the use of TMRM and TMRE quite convenient. TMRE is slightly more hydrophobic than TMRM.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 199.629 µL | 998.143 µL | 1.996 mL | 9.981 mL | 19.963 mL |
| 5 mM | 39.926 µL | 199.629 µL | 399.257 µL | 1.996 mL | 3.993 mL |
| 10 mM | 19.963 µL | 99.814 µL | 199.629 µL | 998.143 µL | 1.996 mL |
Molarity calculator
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Citations
View all 13 citations: Citation Explorer
Chronic calcium signaling in IgE+ B cells limits plasma cell differentiation and survival
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Immunity (2021): 2756--2771
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Immunity (2021): 2756--2771
Genome-wide screens identify calcium signaling as a key regulator of IgE+ plasma cell differentiation and survival
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Available at SSRN 3800380 (2021)
Authors: Newman, Rebecca and Tolar, Pavel
Journal: Available at SSRN 3800380 (2021)
Low-dose sorafenib acts as a mitochondrial uncoupler and ameliorates nonalcoholic steatohepatitis
Authors: Jian, Chongshu and Fu, Jiajun and Cheng, Xu and Shen, Li-Jun and Ji, Yan-Xiao and Wang, Xiaoming and Pan, Shan and Tian, Han and Tian, Song and Liao, Rufang and others,
Journal: Cell metabolism (2020): 892--908
Authors: Jian, Chongshu and Fu, Jiajun and Cheng, Xu and Shen, Li-Jun and Ji, Yan-Xiao and Wang, Xiaoming and Pan, Shan and Tian, Han and Tian, Song and Liao, Rufang and others,
Journal: Cell metabolism (2020): 892--908
A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast
Authors: Li, Yi and Tian, Caiping and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: Cell Chemical Biology (2020)
Authors: Li, Yi and Tian, Caiping and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: Cell Chemical Biology (2020)
Protocol for Proximity-Dependent Proteomic Profiling in Yeast Cells by APEX and Alk-Ph Probe
Authors: Li, Yi and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: STAR protocols (2020): 100137
Authors: Li, Yi and Liu, Keke and Zhou, Ying and Yang, Jing and Zou, Peng
Journal: STAR protocols (2020): 100137
References
View all 73 references: Citation Explorer
High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening
Authors: O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.
Journal: Arch Toxicol (2006): 580
Authors: O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.
Journal: Arch Toxicol (2006): 580
Mitochondrial Ca2+ uptake during simulated ischemia does not affect permeability transition pore opening upon simulated reperfusion
Authors: Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.
Journal: Cardiovasc Res (2006): 715
Authors: Ruiz-Meana M, Garcia-Dorado D, Miro-Casas E, Abellan A, Soler-Soler J.
Journal: Cardiovasc Res (2006): 715
Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation
Authors: Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.
Journal: Eur J Pharmacol (2006): 69
Authors: Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.
Journal: Eur J Pharmacol (2006): 69
NECA at reperfusion limits infarction and inhibits formation of the mitochondrial permeability transition pore by activating p70S6 kinase
Authors: Forster K, Paul I, Solenkova N, Staudt A, Cohen MV, Downey JM, Felix SB, Krieg T.
Journal: Basic Res Cardiol (2006): 319
Authors: Forster K, Paul I, Solenkova N, Staudt A, Cohen MV, Downey JM, Felix SB, Krieg T.
Journal: Basic Res Cardiol (2006): 319
Fatty acid ethyl esters cause pancreatic calcium toxicity via inositol trisphosphate receptors and loss of ATP synthesis
Authors: Criddle DN, Murphy J, Fistetto G, Barrow S, Tepikin AV, Neoptolemos JP, Sutton R, Petersen OH.
Journal: Gastroenterology (2006): 781
Authors: Criddle DN, Murphy J, Fistetto G, Barrow S, Tepikin AV, Neoptolemos JP, Sutton R, Petersen OH.
Journal: Gastroenterology (2006): 781
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