Tide Quencher™ 5.1WS azide [TQ5.1WS azide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1020.08 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 5.1WS amine [TQ5.1WS amine] |
Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide] |
Tide Quencher™ 5.1WS acid [TQ5.1WS acid] |
Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Click Chemistry, Tide Quencher Dyes
Molecular weight 1020.08 |
Tide Quencher™ 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor® 647, Alexa Fluor® 647, iFluor® 647 and iFluor® 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher™ 5.1WS Azide is an excellent building block for preparing TQ5.1WS-labeled probes from alkyne-modified oligonucleotides (including DBCO-modified oligonucleotides) and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ5.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 5.1WS azide [TQ5.1WS azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.032 µL | 490.158 µL | 980.315 µL | 4.902 mL | 9.803 mL |
5 mM | 19.606 µL | 98.032 µL | 196.063 µL | 980.315 µL | 1.961 mL |
10 mM | 9.803 µL | 49.016 µL | 98.032 µL | 490.158 µL | 980.315 µL |
Molarity calculator
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References
View all 50 references: Citation Explorer
Fluorescent Properties of Cyanine Dyes As a Matter of the Environment.
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Journal: Biomacromolecules (2022): 1981-1994
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Journal: Biomacromolecules (2022): 1981-1994
Click Chemistry-Enabled Conjugation Strategy for Producing Dibenzodiazepinone-Type Fluorescent Probes To Target M2 Acetylcholine Receptors.
Authors: Yang, Hongrong and Micovic, Nicholas and Monaghan, James R and Clark, Heather A
Journal: Bioconjugate chemistry (2022): 2223-2233
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An Acidity-Initiated Self-Assembly/Disassembly Nanoprobe to Switch on Fluorescence for Tumor-Targeted Near-Infrared Imaging.
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