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Tide Quencher™ 3 maleimide [TQ3 maleimide]
TQ3 is designed to be a superior quencher to TAMRA, TF3 and Cy3. TQ3 has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ3 product is used for post-labeling of thiol-modified oligonucleotides and and cysteine-containing peptides.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 3 maleimide [TQ3 maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 173.72 µL | 868.598 µL | 1.737 mL | 8.686 mL | 17.372 mL |
| 5 mM | 34.744 µL | 173.72 µL | 347.439 µL | 1.737 mL | 3.474 mL |
| 10 mM | 17.372 µL | 86.86 µL | 173.72 µL | 868.598 µL | 1.737 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Tide Quencher™ 2WS maleimide [TQ2WS maleimide] | 48000 | 0.100 | 0.120 |
| Tide Quencher™ 4WS maleimide [TQ4WS maleimide] | 900001 | 0.149 | 0.136 |
| Tide Quencher™ 5WS maleimide [TQ5WS maleimide] | 130000 | 0.072 | 0.082 |
| Tide Quencher™ 6WS maleimide [TQ6WS maleimide] | 130000 | 0.120 | 0.102 |
| Tide Quencher™ 7WS maleimide [TQ7WS maleimide] | 140000 | 0.072 | 0.091 |
| Tide Quencher™ 1 maleimide [TQ1 maleimide] | 20000 | 0.147 | 0.194 |
| Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å* | 22000 | 0.085 | 0.091 |
| Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å* | 22000 | 0.085 | 0.091 |
| Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite] | 22000 | 0.085 | 0.091 |
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Citations
View all 9 citations: Citation Explorer
Multivalent interactions drive nucleosome binding and efficient chromatin deacetylation by SIRT6
Authors: Liu, Wallace H and Zheng, Jie and Feldman, Jessica L and Klein, Mark A and Kuznetsov, Vyacheslav I and Peterson, Craig L and Griffin, Patrick R and Denu, John M
Journal: Nature communications (2020): 1--13
Authors: Liu, Wallace H and Zheng, Jie and Feldman, Jessica L and Klein, Mark A and Kuznetsov, Vyacheslav I and Peterson, Craig L and Griffin, Patrick R and Denu, John M
Journal: Nature communications (2020): 1--13
Enzymatic amplification of oligonucleotides in paper substrates
Authors: Sedighi, Abootaleb and Krull, Ulrich J
Journal: Talanta (2018)
Authors: Sedighi, Abootaleb and Krull, Ulrich J
Journal: Talanta (2018)
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
References
View all 25 references: Citation Explorer
Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
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