Tide Quencher™ 1 azide [TQ1 azide]

TQ1 is designed to be a superior quencher alternative to DABCYL. Compared to DABCYL, TQ1 have (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ1 product is reactive to alkynes, and useful for click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 1 azide [TQ1 azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	279.002 µL	1.395 mL	2.79 mL	13.95 mL	27.9 mL
5 mM	55.8 µL	279.002 µL	558.005 µL	2.79 mL	5.58 mL
10 mM	27.9 µL	139.501 µL	279.002 µL	1.395 mL	2.79 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
Tide Quencher™ 4WS azide [TQ4WS azide]	90000¹	0.149	0.136
Tide Quencher™ 6WS azide [TQ6WS azide]	130000	0.120	0.102
Tide Quencher™ 1 CPG [TQ1 CPG] 500 Å	20000	0.147	0.194
Tide Quencher™ 1 CPG [TQ1 CPG] 1000 Å	20000	0.147	0.194
Tide Quencher™ 1 phosphoramidite [TQ1 phosphoramidite]	20000	0.147	0.194
Tide Quencher™ 2 azide [TQ2 azide]	21000	0.100	0.12
Tide Quencher™ 3 azide [TQ3 azide]	22000	0.085	0.091
Tide Quencher™ 5WS azide [TQ5WS azide]	130000	0.072	0.082
Tide Quencher™ 7WS azide [TQ7WS azide]	140000	0.072	0.091
Tide Fluor™ 1 azide [TF1 azide]	20000	-	0.187
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Citations

View all 7 citations: Citation Explorer

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16

Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272

References

View all 25 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311

Page updated on December 3, 2024

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Catalog Number	2188
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Physical properties

Molecular weight	358.42
Solvent	DMSO

Spectral properties

Absorbance (nm)	492
Correction Factor (260 nm)	0.147
Correction Factor (280 nm)	0.194
Extinction coefficient (cm ^-1 M ^-1)	20000

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200