Calculator
Tide Fluor™ 6WS alkyne [TF6WS alkyne]
Tide Fluor™ 6 (TF6WS) family has the spectral properties similar to those of Cy5.5, IRDye 700 and Alexa Fluor 680. Their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family more robust to pH-sensitive assays. In some cases TF6-labeled peptides and nucleotides exhibit stronger fluorescence and higher photostability than the ones labeled with Cy5.5, IRDye 700 and Alexa Fluor 680. In pairing with our Tide Quencher™ 6 (TQ6WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF6WS product is reactive to azides, and useful for click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 6WS alkyne [TF6WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 86.041 µL | 430.207 µL | 860.415 µL | 4.302 mL | 8.604 mL |
| 5 mM | 17.208 µL | 86.041 µL | 172.083 µL | 860.415 µL | 1.721 mL |
| 10 mM | 8.604 µL | 43.021 µL | 86.041 µL | 430.207 µL | 860.415 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| Tide Fluor™ 1 alkyne [TF1 alkyne] | 341 | 447 | 20000 | 0.911 | 0.187 |
| Tide Fluor™ 2 alkyne [TF2 alkyne] | 503 | 525 | 75000 | 0.91 | 0.09 |
| Tide Fluor™ 3 alkyne [TF3 alkyne] | 553 | 578 | 750001 | 0.11 | 0.179 |
| Tide Fluor™ 5WS alkyne [TF5WS alkyne] | 649 | 663 | 250000 | 0.271 | 0.027 |
| Tide Fluor™ 6WS acid [TF6WS acid] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.271 | 0.101 |
| Tide Fluor™ 6WS amine [TF6WS amine] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.271 | 0.101 |
| Tide Fluor™ 6WS maleimide [TF6WS maleimide] *Superior replacement for Cy5.5* | 682 | 701 | 220000 | 0.271 | 0.101 |
| Tide Fluor™ 4 alkyne [TF4 alkyne] | 577 | 602 | 90000 | 0.771 | 0.436 |
| Tide Fluor™ 7WS alkyne [TF7WS alkyne] | 756 | 780 | 275000 | 0.31 | 0.049 |
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Citations
View all 8 citations: Citation Explorer
Non-invasive cell tracking of SPIO labeled cells in an intrinsic regenerative environment: The axolotl limb
Authors: Lauridsen, Henrik and Foldager, Casper Bindzus and Hansen, Line and Pedersen, Michael
Journal: Experimental and therapeutic medicine (2018): 3311--3319
Authors: Lauridsen, Henrik and Foldager, Casper Bindzus and Hansen, Line and Pedersen, Michael
Journal: Experimental and therapeutic medicine (2018): 3311--3319
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
References
View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
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