Tide Fluor™ 3WS acid [TF3WS acid] Superior replacement for Cy3

Tide Fluor™ 3WS (TF3WS) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3WS family has better water solubility. It has been used to label the hydrophobic peptides that hasve poor water solubility. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy3. In pairing with our Tide Quencher™ 3 (TQ3), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 3WS acid [TF3WS acid] *Superior replacement for Cy3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	119.205 µL	596.026 µL	1.192 mL	5.96 mL	11.921 mL
5 mM	23.841 µL	119.205 µL	238.41 µL	1.192 mL	2.384 mL
10 mM	11.921 µL	59.603 µL	119.205 µL	596.026 µL	1.192 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
Tide Fluor™ 1 acid [TF1 acid] Superior replacement for EDANS	341	447	20000	0.91¹	0.187
Tide Fluor™ 2 acid [TF2 acid] Superior replacement for fluorescein	503	525	75000	0.9¹	0.09
Tide Fluor™ 3 acid [TF3 acid] Superior replacement for Cy3	553	578	75000¹	0.1¹	0.179
Tide Fluor™ 5WS acid [TF5WS acid] Superior replacement for Cy5	649	663	250000	0.27¹	0.027
Tide Fluor™ 4 acid [TF4 acid] Superior replacement for ROX and Texas Red	577	602	90000	0.77¹	0.436
Tide Fluor™ 6WS acid [TF6WS acid] Superior replacement for Cy5.5	682	701	220000	0.27¹	0.101
Tide Fluor™ 7WS acid [TF7WS acid] Superior replacement for Cy7	756	780	275000	0.3¹	0.049
Tide Fluor™ 8WS acid [TF8WS acid] Near Infrared Emission	785	801	250000	0.21¹	0.109
Tide Fluor™ 2WS acid [TF2WS acid] Superior replacement for FITC	491	516	75000	0.9¹	0.11
Tide Quencher™ 3WS acid [TQ3WS acid]	-	-	90000	-	0.205
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Citations

View all 7 citations: Citation Explorer

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16

Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272

References

View all 25 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311

Page updated on October 28, 2024

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Catalog Number	2345
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Physical properties

Molecular weight	838.89
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.079
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	550
Emission (nm)	563
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501