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Tide Fluor™ 3 alkyne [TF3 alkyne]
Tide Fluor™ 3 (TF3) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy3. TF3-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy3. In pairing with our Tide Quencher™ 3 (TQ3), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF3 product is reactive to azides, and useful for click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 3 alkyne [TF3 alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 201.788 µL | 1.009 mL | 2.018 mL | 10.089 mL | 20.179 mL |
| 5 mM | 40.358 µL | 201.788 µL | 403.576 µL | 2.018 mL | 4.036 mL |
| 10 mM | 20.179 µL | 100.894 µL | 201.788 µL | 1.009 mL | 2.018 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| Tide Fluor™ 1 alkyne [TF1 alkyne] | 341 | 447 | 20000 | 0.911 | 0.187 |
| Tide Fluor™ 2 alkyne [TF2 alkyne] | 503 | 525 | 75000 | 0.91 | 0.09 |
| Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3* | 553 | 578 | 750001 | 0.11 | 0.179 |
| Tide Fluor™ 3 amine [TF3 amine] *Superior replacement for Cy3* | 553 | 578 | 750001 | 0.11 | 0.179 |
| Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3* | 553 | 578 | 750001 | 0.11 | 0.179 |
| Tide Fluor™ 5WS alkyne [TF5WS alkyne] | 649 | 663 | 250000 | 0.271 | 0.027 |
| Tide Fluor™ 4 alkyne [TF4 alkyne] | 577 | 602 | 90000 | 0.771 | 0.436 |
| Tide Fluor™ 6WS alkyne [TF6WS alkyne] | 682 | 701 | 220000 | 0.271 | 0.101 |
| Tide Fluor™ 7WS alkyne [TF7WS alkyne] | 756 | 780 | 275000 | 0.31 | 0.049 |
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Citations
View all 8 citations: Citation Explorer
To what extent do fluorophores bias the biological activity of peptides? A practical approach using membrane-active peptides as models
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
Authors: Cavaco, Marco and P{\'e}rez-Peinado, Clara and Valle, Javier and Silva, R{\'u}ben DM and Correia, Jo{\~a}o DG and Andreu, David and Castanho, Miguel ARB and Neves, Vera
Journal: Frontiers in bioengineering and biotechnology (2020): 552035
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
References
View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
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