Streptavidin-Xtra™ IF555

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

1. Add 100 µL (For Cat# 46002) or 1 mL (For Cat# 46003) of ddH2O to the vial to make a 1 mg/mL stock solution.

   Note: This constituted stock solution will be in PBS with 0.2% BSA.

1. For IF, the recommended staining concentration is 1-5 μg/mL. For FACS, the suggested concentration is 0.1-0.5 μg/100 μL/million cells in the staining buffer.

   Note: PBS + 0.1% BSA can be used as a staining buffer.

   Note: For the best performance of each application, the optimal concentration of this reagent needs to be carefully determined.

   Note: The suggested working dilution is provided as a guide only. It is recommended that the users titrate the product in their tests using proper positive and negative controls.

1. Block and treat the samples with antibodies of interest as per the manufacturer's recommendations.

2. Add biotin-conjugated secondary antibody working solution in the samples at appropriate concentration and duration.

   Note: Please verify the compatibility and type of your biotin-conjugated antibody with the primary antibody used in the experiment. For example, If the primary antibody is a mouse antibody, then a goat Anti-Mouse antibody bound with biotin can be used for the assay.

3. Incubate the cells with Streptavidin-Xtra™ working solution at room temperature for 30 minutes to 1 hour. Note: Optimal time for incubation needs to be determined carefully.
4. Remove the working solution and resuspend the cells in your choice of buffer.
5. Take the image using the fluorescence microscope or record the intensity using flow cytometer.