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RPE-Cy7-streptavidin conjugate

Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This RPE-Cy7-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with RPE-Cy7 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy7® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
RPE-Cy5-streptavidin conjugate65167025000010.271, 0.420.020.030.0090.09

Citations

View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996

References

View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Page updated on March 31, 2025

Ordering information

Price
Unit size
100 ug
1 mg
Catalog Number
1691616917
Quantity
Add to cart

Additional ordering information

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Physical properties

Molecular weight

~292000

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

1960000

Excitation (nm)

565

Emission (nm)

778

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature.

Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by RPE–Cy7–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the RPE–Cy7-specific B13-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature.

Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by RPE–Cy7–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the RPE–Cy7-specific B13-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature.

Bottom) Flow cytometry analysis of whole blood cells stained first with CD4–biotin followed by RPE–Cy7–streptavidin conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the RPE–Cy7-specific B13-A channel.
HL-60 cells were incubated with (Red, +) or without (Green, -) biotinylated-Anti-human HLA-ABC (W6/32 mAb-Biotin), followed by Streptavidin-PE/Cy7 stain. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in PE-Cy7 channel.