Name: RPE-Cy5-streptavidin conjugate
Brand: AAT Bioquest
SKU: 16918
Availability: InStock

RPE-Cy5-streptavidin conjugate

Streptavidin conjugates are widely used together with a biotin conjugate for specific detection of a variety of biological molecules since streptavidin has a very high binding affinity for biotin and its conjugates. RPE-Cy5-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with RPE-Cy5 covalently attached (as the fluorescent label). It is used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy5® filter set. AAT Bioquest offers the largest selection of biotinylated and streptavidin conjugates and reagents. Some of them are not available from other commercial vendors.

PBMC cells were stained with (Red) or without (Green) mouse anti-CD45-Biotin and then followed with PE-Cy5-streptavidin conjugate stain. <br />Blue: Unstained cells.

Protocol

COA

Catalog	Size	Price	Quantity
16918	100 ug	Price
16919	1 mg	Price

References

View all 7 references: Citation Explorer

Tumor Extracellular pH-Driven Cancer-Selective Artificial Receptor-Mediated Tumor-Targeted Fluorescence Imaging.

Authors:

Guo, Qian and Wu, Zhan and Peng, Yongbo and Peng, Wenyi and Huang, Qin and Peng, Miao and Huang, Ni and Hu, Xiaoxiao and Fu, Ting and Zhao, Zilong and Tan, Weihong

Journal:

Analytical chemistry (2019): 13349-13354

Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion.

Authors:

Liu, Xiaoxia and Seven, Alpay Burak and Xu, Junjie and Esser, Victoria and Su, Lijing and Ma, Cong and Rizo, Josep

Journal:

Nature protocols (2017): 2014-2028

Optimal Structure of a Plasmonic Chip for Sensitive Bio-Detection with the Grating-Coupled Surface Plasmon-Field Enhanced Fluorescence (GC-SPF).

Authors:

Tawa, Keiko and Nakayama, Takuya and Kintaka, Kenji

Journal:

Materials (Basel, Switzerland) (2017)

Targeting prostate cancer cells with a multivalent PSMA inhibitor-guided streptavidin conjugate.

Authors:

Liu, Tiancheng and Nedrow-Byers, Jessie R and Hopkins, Mark R and Wu, Lisa Y and Lee, Jeonghoon and Reilly, Peter T A and Berkman, Clifford E

Journal:

Bioorganic & medicinal chemistry letters (2012): 3931-4

Use of a multiplexed CMOS microarray to optimize and compare oligonucleotide binding to DNA probes synthesized or immobilized on individual electrodes.

Authors:

Maurer, Karl and Yazvenko, Nina and Wilmoth, Jodi and Cooper, John and Lyon, Wanda and Danley, David

Journal:

Sensors (Basel, Switzerland) (2010): 7371-85

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.02
Correction factor (280 nm)	0.03
Correction factor (482 nm)	0.009
Correction factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27 ¹ , 0.4 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 14, 2025