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Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

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Rhod-4™, sodium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Rhod-4™, sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.603 µL613.016 µL1.226 mL6.13 mL12.26 mL
5 mM24.521 µL122.603 µL245.206 µL1.226 mL2.452 mL
10 mM12.26 µL61.302 µL122.603 µL613.016 µL1.226 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Product family

NameExcitation (nm)Emission (nm)Quantum yield
Rhod-4™, potassium salt5235510.11

Citations

View all 23 citations: Citation Explorer
Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient
Authors: Shimojima, Masaya and Yuasa, Shinsuke and Motoda, Chikaaki and Yozu, Gakuto and Nagai, Toshihiro and Ito, Shogo and Lachmann, Mark and Kashimura, Shin and Takei, Makoto and Kusumoto, Dai and others, undefined
Journal: Scientific Reports (2017)
Preliminary findings on ultrasound modulation of the electromechanical function of human stem-cell-derived cardiomyocytes
Authors: Chen, Andrew William and Klimas, Aleks and ra , undefined and Zderic, Vesna and Castellanos, Ivan Suares and Entcheva, Emilia
Journal: (2017): 1--4
The role of spatial organization of Ca (2+) release sites in the generation of arrhythmogenic diastolic Ca (2+) release in myocytes from failing hearts.
Authors: Belevych, Andriy E and Ho, Hsiang-Ting and Bonilla, Ingrid M and Terentyeva, Radmila and Schober, Karsten E and Terentyev, Dmitry and Carnes, Cynthia A and Györke, Sándor
Journal: Basic research in cardiology (2017): 44
Individual evaluation of cardiac marker expression and self-beating during cardiac differentiation of P19CL6 cells on different culture substrates
Authors: Yamaoka, Tetsuji and Hirata, Mitsuhi and Dan, Takaaki and Yamashita, Atsushi and Otaka, Akihisa and Nakaoki, Takahiko and Miskon, Azizi and Kakinoki, Sachiro and Mahara, Atsushi
Journal: Journal of Biomedical Materials Research Part A (2016)
Page updated on November 21, 2024

Ordering information

Price
Unit size
1 mg
5x50 ug
Catalog Number
2111821128
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Dissociation constant (Kd, nM)451

Molecular weight

815.64

Solvent

Water

Spectral properties

Excitation (nm)

523

Emission (nm)

551

Quantum yield

0.11

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
ATP-stimulated calcium responses of endogenous P2Y receptors were measured in CHO-K1 cells with Rhod-4&trade; AM (Cat# 21120) and Rhod-2 AM (Cat# 21064). CHO-K1 cells were seeded overnight at 50,000 cells/100 &micro;L/well in a Costar 96-well black wall/clear bottom plate. The growth medium was removed, and the cells were incubated with 100 &micro;L of dye loading solution using Rhod-4&trade; AM (4 &micro;M, A and B) or Rhod-2 AM (4 &micro;M, C and D) for 1 hour in a 37 &deg;C, 5% CO2 incubator. The staining solution was replaced with 200 &micro;L HHBS, then the cells were imaged before (A and C) and after (B and D) ATP treatment with a fluorescence microscope (Olympus IX71) using TRITC channel.
ATP-stimulated calcium responses of endogenous P2Y receptors were measured in CHO-K1 cells with Rhod-4&trade; AM (Cat# 21120) and Rhod-2 AM (Cat# 21064). CHO-K1 cells were seeded overnight at 50,000 cells/100 &micro;L/well in a Costar 96-well black wall/clear bottom plate. The growth medium was removed, and the cells were incubated with 100 &micro;L of dye loading solution using Rhod-4&trade; AM (4 &micro;M, A and B) or Rhod-2 AM (4 &micro;M, C and D) for 1 hour in a 37 &deg;C, 5% CO2 incubator. The staining solution was replaced with 200 &micro;L HHBS, then the cells were imaged before (A and C) and after (B and D) ATP treatment with a fluorescence microscope (Olympus IX71) using TRITC channel.
ATP-stimulated calcium responses of endogenous P2Y receptors were measured in CHO-K1 cells with Rhod-4&trade; AM (Cat# 21120) and Rhod-2 AM (Cat# 21064). CHO-K1 cells were seeded overnight at 50,000 cells/100 &micro;L/well in a Costar 96-well black wall/clear bottom plate. The growth medium was removed, and the cells were incubated with 100 &micro;L of dye loading solution using Rhod-4&trade; AM (4 &micro;M, A and B) or Rhod-2 AM (4 &micro;M, C and D) for 1 hour in a 37 &deg;C, 5% CO2 incubator. The staining solution was replaced with 200 &micro;L HHBS, then the cells were imaged before (A and C) and after (B and D) ATP treatment with a fluorescence microscope (Olympus IX71) using TRITC channel.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Rhodamines and Rhodamine Derivatives
Rhod-4™, AM
Rhod-4™ amine
Rhod-4™ azide