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Resazurin, sodium salt *CAS 62758-13-8*
Resazurin is a non-toxic, water-soluble and redox-sensitive dye that changes from its blue/non-fluorescent state to pink/highly-fluorescent upon reduction to resorufin by many biological processes. Resazurin added to viable cells become reduced via an as yet unknown mechanism by enzymatic or chemical reactions in cells. This feature is widely used for measuring cell proliferation, viability and cytotoxicity. Resazurin may be added at any time during the culture period. For measurement of cell proliferation, it is best to add resazurin during log phase growth. Incubation times may vary depending on the metabolic rates of the cell lines being tested. Fluorescence can be read using 544 nm/590 nm filter set. Absorbance can be read using a spectrophotometer set at 570 nm.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Resazurin, sodium salt *CAS 62758-13-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 398.137 µL | 1.991 mL | 3.981 mL | 19.907 mL | 39.814 mL |
| 5 mM | 79.627 µL | 398.137 µL | 796.273 µL | 3.981 mL | 7.963 mL |
| 10 mM | 39.814 µL | 199.068 µL | 398.137 µL | 1.991 mL | 3.981 mL |
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| Resorufin, sodium salt *CAS 34994-50-8* | 571 | 584 | 650001 | 0.751 | - |
| EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0* | 336 | 455 | 5900 | - | 0.107 |
Citations
View all 1 citations: Citation Explorer
Digital Cascade Assays for ADP-or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device
Authors: Ueno, Hiroshi and Sano, Mio and Hara, Mayu and Noji, Hiroyuki
Journal: ACS sensors (2023)
Authors: Ueno, Hiroshi and Sano, Mio and Hara, Mayu and Noji, Hiroyuki
Journal: ACS sensors (2023)
References
View all 65 references: Citation Explorer
Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers
Authors: Nateche F, Martin A, Baraka S, Palomino JC, Khaled S, Portaels F.
Journal: J Med Microbiol (2006): 857
Authors: Nateche F, Martin A, Baraka S, Palomino JC, Khaled S, Portaels F.
Journal: J Med Microbiol (2006): 857
Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis
Authors: Umubyeyi AN, Martin A, Zissis G, Struelens M, Karita E, Portaels F.
Journal: Int J Tuberc Lung Dis (2006): 808
Authors: Umubyeyi AN, Martin A, Zissis G, Struelens M, Karita E, Portaels F.
Journal: Int J Tuberc Lung Dis (2006): 808
A fluorometric study of relative ocular lens cytosensitivity to multipurpose contact lens solutions using the resazurin assay method
Authors: Oriowo MO., undefined
Journal: Toxicol In Vitro (2006): 1548
Authors: Oriowo MO., undefined
Journal: Toxicol In Vitro (2006): 1548
Development and application of a resazurin-based biomass activity test for activated sludge plant management
Authors: McNicholl BP, McGrath JW, Quinn JP.
Journal: Water Res. (2006)
Authors: McNicholl BP, McGrath JW, Quinn JP.
Journal: Water Res. (2006)
A resazurin-based biosensor for organic pollutants
Authors: Tizzard AC, Bergsma JH, Lloyd-Jones G.
Journal: Biosens Bioelectron (2006): 759
Authors: Tizzard AC, Bergsma JH, Lloyd-Jones G.
Journal: Biosens Bioelectron (2006): 759
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