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ReadiUse™ WST-8 *50 mM aqueous solution*
WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor, 1-methoxy phenazine methosulfate, NADPH-dependent cellular oxidoreductases, acting via plasma membrane electron transport, reduce the colorless cell-impermeant WST-8 outside the cell to a water-soluble orange formazan dye with absorbance max at 460 nm. WST-8 is very stable, and tolerates a long time incubation (such as 24 to 48 hours) due to its little cytotoxicity. WST-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS. It is typically used as a cell viability indicator in cell proliferation assays.
Citations
View all 6 citations: Citation Explorer
Establishment, culture and characterization of gemcitabine hydrochloride-resistant human non-small cell lung carcinoma cell line derived cancer stem cells
Authors: Demir Cevizlidere, Bahar and Uysal, Onur and Avci, Huseyin and Gunes Bagis, Sibel and Semerci Sevimli, Tugba and Dincer, Murat and Qomi Ekenel, Emilia and Kara, Suleyman Gokhan and Soykan, Merve Nur and Eker Sariboyaci, Ayla
Journal: Cell Biochemistry and Function (2024): e4079
Authors: Demir Cevizlidere, Bahar and Uysal, Onur and Avci, Huseyin and Gunes Bagis, Sibel and Semerci Sevimli, Tugba and Dincer, Murat and Qomi Ekenel, Emilia and Kara, Suleyman Gokhan and Soykan, Merve Nur and Eker Sariboyaci, Ayla
Journal: Cell Biochemistry and Function (2024): e4079
Cytotoxicity effect and transcriptome analysis of PCV3-infected cells revealed potential viral pathogenic mechanisms
Authors: Chang, Chia-Chun and Wu, Ching-Ying and Wu, Chi-Ming and Wu, Ching-Wei and Wang, Yi-Chen and Lin, Guang-Jan and Chien, Maw-Sheng and Huang, Chienjin
Journal: Microbial Pathogenesis (2024): 106715
Authors: Chang, Chia-Chun and Wu, Ching-Ying and Wu, Chi-Ming and Wu, Ching-Wei and Wang, Yi-Chen and Lin, Guang-Jan and Chien, Maw-Sheng and Huang, Chienjin
Journal: Microbial Pathogenesis (2024): 106715
Exploring the surface epitope and nuclear localization analysis of porcine circovirus type 3 capsid protein
Authors: Chang, Chia-Chun and Wu, Ching-Ying and Ciou, Jhao-Guan and Wu, Ching-Wei and Wang, Yi-Chen and Chang, Hui-Wen and Chien, Maw-Sheng and Huang, Chienjin
Journal: AMB Express (2023): 1--11
Authors: Chang, Chia-Chun and Wu, Ching-Ying and Ciou, Jhao-Guan and Wu, Ching-Wei and Wang, Yi-Chen and Chang, Hui-Wen and Chien, Maw-Sheng and Huang, Chienjin
Journal: AMB Express (2023): 1--11
miR-218 inhibits the proliferation of human glioma cells through downregulation of Yin Yang 1
Authors: Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng
Journal: Molecular Medicine Reports (2018): 1926--1932
Authors: Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng
Journal: Molecular Medicine Reports (2018): 1926--1932
MiR-218 suppresses osteosarcoma proliferation by down-regulating BMI1
Authors: Xuan, Chengmin and Jin, Mingwei and Gao, Yong and Xu, Shumei and Wang, Lei and Wang, Yuan and Han, Rui and An, Qi
Journal: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2017): 6342--6350
Authors: Xuan, Chengmin and Jin, Mingwei and Gao, Yong and Xu, Shumei and Wang, Lei and Wang, Yuan and Han, Rui and An, Qi
Journal: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2017): 6342--6350
References
View all 5 references: Citation Explorer
A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line
Authors: Tiwari, K.; Wavdhane, M.; Haque, S.; Govender, T.; Kruger, H. G.; Mishra, M. K.; Ch and ra, R.; Tiwari, D.
Journal: Pharm Biol (2015): 849-54
Authors: Tiwari, K.; Wavdhane, M.; Haque, S.; Govender, T.; Kruger, H. G.; Mishra, M. K.; Ch and ra, R.; Tiwari, D.
Journal: Pharm Biol (2015): 849-54
Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria
Authors: Tsukatani, T.; Suenaga, H.; Shiga, M.; Noguchi, K.; Ishiyama, M.; Ezoe, T.; Matsumoto, K.
Journal: J Microbiol Methods (2012): 160-6
Authors: Tsukatani, T.; Suenaga, H.; Shiga, M.; Noguchi, K.; Ishiyama, M.; Ezoe, T.; Matsumoto, K.
Journal: J Microbiol Methods (2012): 160-6
WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells
Authors: Stoddart, M. J.
Journal: Methods Mol Biol (2011): 21-5
Authors: Stoddart, M. J.
Journal: Methods Mol Biol (2011): 21-5
monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates
Authors: Brady, A. J.; Kearney, P.; Tunney, M. M., Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium
Journal: J Microbiol Methods (2007): 305-11
Authors: Brady, A. J.; Kearney, P.; Tunney, M. M., Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium
Journal: J Microbiol Methods (2007): 305-11
Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency
Authors: Arai, M.; Kosuge, K.; Kawamoto, F.; Matsuoka, H.
Journal: Acta Med Okayama (2006): 127-34
Authors: Arai, M.; Kosuge, K.; Kawamoto, F.; Matsuoka, H.
Journal: Acta Med Okayama (2006): 127-34
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