Pyronin Y CAS 92-32-0

Pyronin Y is widely used in combination with methyl green to selectively and differentially stain nucleic acids following polyacrylamide gel electrophoresis. In a dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains DNA green.

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Citations

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Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis
Authors: Zielke, Claudia and Pan, Ching W and Gutierrez Ramirez, Adriana J and Feit, Cameron and Dobson, Chandler and Davidson, Catherine and Sandel, Brody and Abbyad, Paul
Journal: Analytical Chemistry (2020): 6949--6957

References

View all 34 references: Citation Explorer

A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)

Methyl green-pyronin Y staining of nucleic acids: studies on the effects of staining time, dye composition and diffusion rates
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27

Pyronin Y as a fluorescent stain for paraffin sections
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299

Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109

Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193

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Physical properties

Molecular weight	302.8
Solvent	DMSO

Spectral properties

Absorbance (nm)	550
Excitation (nm)	547
Emission (nm)	566

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	92-32-0