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Protonex™ NIR 770 NHS ester

Protonex™ NIR 770 NHS ester is the only cell-permeable acidotropic pH dye commercially available for developing NIR pH probes. It is an excellent building block that can be readily used for developing site-specific pH sensors for monitoring pH fluctuation in a spatial environment. It can be readily conjugated to amino-containing biomolecules (such as antibodies, peptides and amino-modified oligos). It easily penetrates live cell membranes, thus can be used for monitoring pH changes in live cells. It is complimentary to CypHer7E that is cell-impermeable, and often used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. Protonex™ NIR 770 NHS ester is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events. It is one of the very few acidotropic pH probes that exhibit NIR fluorescence.

Spectrum

References

View all 50 references: Citation Explorer
Fisetin-Mediated Perturbations of Membrane Permeability and Intracellular pH in Candida albicans.
Authors: Kim, Younhee
Journal: Journal of microbiology and biotechnology (2024): 1-10
Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe.
Authors: Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique
Journal: Biomolecules (2023)
mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome.
Authors: Kashirina, Alena and Gavrina, Alena and Kryukov, Emil and Elagin, Vadim and Kolesova, Yuliya and Artyuhov, Alexander and Momotyuk, Ekaterina and Abdyyev, Vepa and Meshcheryakova, Natalia and Zagaynova, Elena and Dashinimaev, Erdem and Kashina, Aleksandra
Journal: Biomedicines (2021)
Plastidial transporters KEA1 and KEA2 at the inner envelope membrane adjust stromal pH in the dark.
Authors: Aranda Sicilia, María Nieves and Sánchez Romero, María Elena and Rodríguez Rosales, María Pilar and Venema, Kees
Journal: The New phytologist (2021): 2080-2090
Page updated on February 12, 2025

Ordering information

Price
Unit size
Catalog Number21221
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Request quotation

Physical properties

Molecular weight

535.64

Solvent

DMSO

Spectral properties

Excitation (nm)

748

Emission (nm)

769

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
Image of Hela cells stained with Protonex™ NIR 770 NHS ester. Hela cells were washed 3 times with HH buffer and then incubated with 10 µM of Protonex™ NIR 770 NHS ester in HH buffer for 30 min at 37°C. The cells were then washed 3 times with HH buffer and incubated with HH buffer or pH=5.0 and pH=8.0 buffer (Cat# 21235) containing 10 µM of nigericin for 5 min at 37°C. Images were acquired using a fluorescence microscope equipped with a Cy7 filter set.
Image of Hela cells stained with Protonex™ NIR 770 NHS ester. Hela cells were washed 3 times with HH buffer and then incubated with 10 µM of Protonex™ NIR 770 NHS ester in HH buffer for 30 min at 37°C. The cells were then washed 3 times with HH buffer and incubated with HH buffer or pH=5.0 and pH=8.0 buffer (Cat# 21235) containing 10 µM of nigericin for 5 min at 37°C. Images were acquired using a fluorescence microscope equipped with a Cy7 filter set.
Image of Hela cells stained with Protonex™ NIR 770 NHS ester. Hela cells were washed 3 times with HH buffer and then incubated with 10 µM of Protonex™ NIR 770 NHS ester in HH buffer for 30 min at 37°C. The cells were then washed 3 times with HH buffer and incubated with HH buffer or pH=5.0 and pH=8.0 buffer (Cat# 21235) containing 10 µM of nigericin for 5 min at 37°C. Images were acquired using a fluorescence microscope equipped with a Cy7 filter set.

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Amine Reactive Dyes and Probes for Conjugation
Protonex™ Red 600
Protonex™ Red 600, SE
Protonex™ Red 600-Latex Bead Conjugate