Protonex™ Blue 450 NHS ester
Protonex™ Blue 450 NHS ester is an amine-reactive pH probe that can be readily conjugated to a variety of biological targets via their primary amino groups. Protonex™ Blue 450 dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Blue 450 dye. The fluorescence of Protonex™ Blue 450 dye increases as pH decreases from neutral to the acidic. The weak fluorescence outside the cell may potentially eliminate the wash steps. Protonex™ Blue 450 dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex™ Blue 450 dye is weakly fluorescent outside the cells, but its fluorescence is significantly enhanced in acidic compartments (such as phagosomes, lysosomes and endosomes). Protonex™ Blue 450 enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. It can be also used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies.
References
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Ternary recognition fluorescent probe for lysosome acidification counter-ion studies via Cl-, K+, and pH.
Authors: Zou, Guang-Yue and Bi, Fan and Chen, Shuai and Liu, Meng-Xian and Yu, Yong-Liang
Journal: Chemical communications (Cambridge, England) (2024)
Authors: Zou, Guang-Yue and Bi, Fan and Chen, Shuai and Liu, Meng-Xian and Yu, Yong-Liang
Journal: Chemical communications (Cambridge, England) (2024)
Protocol for qualitative analysis of lysosome immunoprecipitation from patient-derived glioblastoma stem-like cells.
Authors: Maghe, Clément and Gavard, Julie
Journal: STAR protocols (2024): 103121
Authors: Maghe, Clément and Gavard, Julie
Journal: STAR protocols (2024): 103121
Peptide-Directed Synthesis of Aggregation-Induced Emission Enhancement-Active Gold Nanoclusters for Single- and Two-Photon Imaging of Lysosome and Expressed αvβ3 Integrin Receptors.
Authors: Madhu, Manivannan and Tseng, Wei-Bin and Chou, Yi-Shiuan and Krishna Kumar, A Santhana and Lu, Chi-Yu and Chang, Po-Ling and Tseng, Wei-Lung
Journal: Analytical chemistry (2024): 9007-9015
Authors: Madhu, Manivannan and Tseng, Wei-Bin and Chou, Yi-Shiuan and Krishna Kumar, A Santhana and Lu, Chi-Yu and Chang, Po-Ling and Tseng, Wei-Lung
Journal: Analytical chemistry (2024): 9007-9015
A novel pH-activated AIEgen probe for dynamic lysosome tracking and high-efficiency photodynamic therapy.
Authors: Hu, Yingcai and Yin, Sheng-Yan and Deng, Ting and Li, Jishan
Journal: Chemical communications (Cambridge, England) (2024): 3047-3050
Authors: Hu, Yingcai and Yin, Sheng-Yan and Deng, Ting and Li, Jishan
Journal: Chemical communications (Cambridge, England) (2024): 3047-3050
A novel probe to monitor lysosome-mitochondria contact sites opens up a new path to study neurodegenerative diseases.
Authors: Makino, Mai and Nakamura, Shuhei
Journal: Cell calcium (2024): 102887
Authors: Makino, Mai and Nakamura, Shuhei
Journal: Cell calcium (2024): 102887