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ProLite™ His-Tag Protein Gel Staining Kit *Green Fluorescence*
The polyhistidine tag (His-Tag) is widely used for protein purification, detection, and immobilization. The ProLite™ His-Tag Protein Gel Staining Kit provides a fast, sensitive, and highly specific fluorescent stain for visualizing His-tagged fusion proteins directly in a polyacrylamide gel following electrophoresis. This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter. It detects nanogram of His-tagged proteins directly in gels, thus eliminating the additional western blotting step. AAT Bioquest offers the largest selection of the His-Tag detection and purification products.
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
ProLite™ His-Tag Protein Gel Stain stock solution
Prepare a ProLite™ His-Tag Protein Gel Stain stock solution by adding 60 µL of DMSO to a vial of ProLite™ His-Tag Protein Gel Stain. Note: 1 vial of stock solution is suitable for two gels. Store any unused stock solution at -20 °C.PREPARATION OF WORKING SOLUTION
ProLite™ His Tag Protein Gel Stain working solution
Prepare a ProLite™ His-Tag Protein Gel Stain working solution by adding 30 µL of ProLite™ His-Tag Protein Gel Stain stock solution to 30 mL of PBS. Note: Make enough working solution that the gel is completely immersed in the working solution. Do not reuse the working solution.SAMPLE EXPERIMENTAL PROTOCOL
The following protocol should be used as a guideline.
Post-staining protocol
- Run gels based on your standard protocol.
- Place the gel in a suitable container, and fix the gel in a fixing solution for 60 minutes.
Note: 40% ethanol + 10% acetic acid can be used as a fixing solution.
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Wash the gel twice with ultra-pure water.
- Incubate the gel in the ProLite™ His-Tag Protein Gel Stain working solution for 60 minutes. Be sure the gel is immersed in the working solution.
- Remove the working solution and wash the gel twice with PBS.
- Proceed to imaging the gel immediately.
Spectrum
References
View all 43 references: Citation Explorer
Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair.
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223
A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654
The surface syndecan protein from Macrobrachium rosenbergii could function as mediator in bacterial infections.
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68
[Cloning and expression of SmDXS2 gene in Swertia mussotii].
Authors: Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941
Authors: Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941
Overexpression, Purification and Functional Characterisation of Wild-Type HIV-1 Subtype C Protease and Two Variants Using a Thioredoxin and His-Tag Protein Fusion System.
Authors: Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien
Journal: The protein journal (2018): 369-379
Authors: Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien
Journal: The protein journal (2018): 369-379
Page updated on November 23, 2024
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