PE/XFD610 Anti-human CD45 Antibody *UCHL1, XFD610 Same Structure to Alexa Fluor™ 610*

UCHL1 is an anti-human monoclonal antibody that forms an immune complex with the CD45ro antigen. CD45ro (sometimes called CD45RO) is a 180 kD transmembrane glycoprotein that is found on the surface of cells like granulocytes, macrophages and NK cells. In certain organisms, CD45 plays a role in the upregulation of protein kinase activity, is a positive regulator of tumor necrosis factor production and is a negative regulator of protein kinase activity. Additionally, it has been associated with key biological processes like dephosphorylation, particularly protein dephosphorylation. CD45 acts in key cellular pathways, for example, the regulation of receptor signaling pathway via JAK-STAT, B cell receptor signaling pathway and positive regulation of extrinsic apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like Src kinases, p56lck and p59fyn. CD45 is a very popular antibody target, with over 54000 publications in the last decade. CD45ro is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of inhibitory molecules and immunology. This antibody was purified through affinity chromatography and conjugated to PE/XFD610 (ex/em = 567/627 nm). XFD610 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 610 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 561 nm laser and 610/20 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Page updated on December 17, 2024