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AAT Bioquest

PE-iFluor® 660 Tandem

Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 660 is a new unique tandem color for multicolor applications, in particular, for designing spectral flow cytometric panels. It has been validated with Cytek’s Aurora flow cytometer. AAT Bioquest also offers a unique preactivated PE-iFluor® 660 to facilitate the PE-iFluor® 660 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 660 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 780 Tandem5665751960000
PE-iFluor® 597 Tandem565612-
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 770 Tandem5675751960000
Show More (2)

References

View all 35 references: Citation Explorer
Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.
Authors: Kreutmair, Stefanie and Unger, Susanne and Núñez, Nicolás Gonzalo and Ingelfinger, Florian and Alberti, Chiara and De Feo, Donatella and Krishnarajah, Sinduya and Kauffmann, Manuel and Friebel, Ekaterina and Babaei, Sepideh and Gaborit, Benjamin and Lutz, Mirjam and Jurado, Nicole Puertas and Malek, Nisar P and Goepel, Siri and Rosenberger, Peter and Häberle, Helene A and Ayoub, Ikram and Al-Hajj, Sally and Nilsson, Jakob and Claassen, Manfred and Liblau, Roland and Martin-Blondel, Guillaume and Bitzer, Michael and Roquilly, Antoine and Becher, Burkhard
Journal: Immunity (2021): 1578-1593.e5
Protocol for Creating Antibodies with Complex Fluorescence Spectra.
Authors: McCarthy, Madeline E and Anglin, Caitlin M and Peer, Heather A and Boleman, Sevanna A and Klaubert, Stephanie R and Birtwistle, Marc R
Journal: Bioconjugate chemistry (2021): 1156-1166
Methotrexate Hampers Immunogenicity to BNT162b2 mRNA COVID-19 Vaccine in Immune-Mediated Inflammatory Disease.
Authors: Haberman, Rebecca H and Herati, Ramin Sedaghat and Simon, David and Samanovic, Marie and Blank, Rebecca B and Tuen, Michael and Koralov, Sergei B and Atreya, Raja and Tascilar, Koray and Allen, Joseph R and Castillo, Rochelle and Cornelius, Amber R and Rackoff, Paula and Solomon, Gary and Adhikari, Samrachana and Azar, Natalie and Rosenthal, Pamela and Izmirly, Peter and Samuels, Jonathan and Golden, Brian and Reddy, Soumya and Neurath, Markus and Abramson, Steven B and Schett, Georg and Mulligan, Mark J and Scher, Jose U
Journal: medRxiv : the preprint server for health sciences (2021)
Normal Numbers of Stem Cell Memory T Cells Despite Strongly Reduced Naive T Cells Support Intact Memory T Cell Compartment in Ataxia Telangiectasia.
Authors: Weitering, Thomas J and Melsen, Janine E and van Ostaijen-Ten Dam, Monique M and Weemaes, Corry M R and Schilham, Marco W and van der Burg, Mirjam
Journal: Frontiers in immunology (2021): 686333
Human Lung-Resident Macrophages Colocalize with and Provide Costimulation to PD1hi Tissue-Resident Memory T Cells.
Authors: Snyder, Mark E and Sembrat, John and Noda, Kentaro and Myerburg, Michael M and Craig, Andrew and Mitash, Nilay and Harano, Takashi and Furukawa, Masashi and Pilewski, Joseph and McDyer, John and Rojas, Mauricio and Sanchez, Pablo
Journal: American journal of respiratory and critical care medicine (2021): 1230-1244
Page updated on November 21, 2024

Ordering information

Price
Unit size
Catalog Number2602
Quantity
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Additional ordering information

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Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)

566

Extinction coefficient (cm -1 M -1)

1960000

Excitation (nm)

565

Emission (nm)

695

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.
Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.
Flow cytometry analysis of PBMC stained with PE-iFlour® 660 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 660 specific B9-A channel.
The fluorescence intensity of PE-iFluor® 660 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.