logo
AAT Bioquest

PE-iFluor® 594 Tandem

Product key features

  • High Brightness & Photostability - Delivers strong, stable fluorescence (Ex/Em: 565/606 nm), enabling sensitive detection of low-abundance targets.
  • Optimized for Flow Cytometers - Efficient excitation with 488/561 nm lasers; compatible with 610/20 nm filters for streamlined multicolor panel design.
  • Enhanced Signal Resolution - Higher quantum yield and lower background than PE-Texas Red, improving signal-to-noise ratios and reducing spectral spillover.

Product description

PE-iFluor® 594 is a high-performance tandem fluorophore optimized for flow cytometry, immunophenotyping, and fluorescence-activated cell sorting (FACS). Upon covalent attachment to antibodies or proteins via maleimide-activated crosslinkers such as SMCC, it enables precise detection of cellular markers in complex, multicolor panels.

This tandem dye combines R-phycoerythrin (PE) as the donor and iFluor® 594 as the acceptor, yielding a bright, photostable emission profile with excitation and emission maxima at 565 nm and 606 nm, respectively. PE-iFluor® 594 is efficiently excited by both 488 nm and 561 nm laser lines and is compatible with standard 610/20 nm bandpass filters, facilitating integration into conventional cytometry systems.

Compared to traditional PE-Texas Red conjugates, PE-iFluor® 594 offers enhanced fluorescence intensity, improved quantum yield, and lower background, resulting in superior signal-to-noise ratios and reduced spectral overlap—critical parameters for high-dimensional cytometry. Its robust photostability ensures consistent performance during extended acquisition, enabling accurate detection of low-abundance targets and rare cell populations.

For added convenience, PE-iFluor® 594 is also available in a ReadiUse® preactivated format. Pre-functionalized with Buccutite® FOL chemistry, it enables efficient, thiol-free conjugation. In this system, the target antibody or protein is first modified with Buccutite® MTA, then reacted with the FOL-activated PE-iFluor® 594, producing stable, bioactive conjugates in a rapid, aqueous workflow, ready for immediate use in flow cytometry applications.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 597 Tandem565612-
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 660 Tandem5656951960000
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 770 Tandem5675751960000
PE-iFluor® 780 Tandem5665751960000
Show More (2)

References

View all 8 references: Citation Explorer
Orange juice and its major polyphenol hesperidin consumption do not induce immunomodulation in healthy well-nourished humans.
Authors: Perche, Olivier and Vergnaud-Gauduchon, Juliette and Morand, Christine and Dubray, Claude and Mazur, Andrzej and Vasson, Marie-Paule
Journal: Clinical nutrition (Edinburgh, Scotland) (2014): 130-5
Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity.
Authors: Fromm, Jonathan R and Thomas, Anju and Wood, Brent L
Journal: American journal of clinical pathology (2009): 322-32
Optimization of three- and four-color multiparameter DNA analysis in lymphoma specimens.
Authors: Plander, M and Brockhoff, G and Barlage, S and Schwarz, S and Rothe, G and Knuechel, R
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2003): 66-74
Multiparameter cytokine-specific affinity matrix assay for the determination of frequencies and phenotype of antigen-reactive T cells.
Authors: Mathioudakis, George and Coder, David and Fefer, Alexander
Journal: Journal of immunological methods (2002): 37-42
Conjugation of fluorochromes to monoclonal antibodies.
Authors: Holmes, K L and Lantz, L M and Russ, W
Journal: Current protocols in cytometry (2001): Unit 4.2
Page updated on April 25, 2025

Ordering information

Price
Unit size
Catalog Number2600
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

~240000

Solvent

Water

Spectral properties

Absorbance (nm)

566

Extinction coefficient (cm -1 M -1)

1960000

Excitation (nm)

565

Emission (nm)

606

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with PE/iFlour® 594 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 594 specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with PE/iFlour® 594 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 594 specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood stained with PE/iFlour® 594 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 594 specific B6-A channel.
Stain index comparison of CD4+ signal using fluorophore-labeled antibody conjugates. Human peripheral blood mononuclear cells (PBMCs) were isolated and stained using AAT Bioquest PE/iFluor® 594 anti-human CD4 conjugates or Biolegend PE/Dazzle™ 594 anti-human CD conjugates. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 594 specific B6-A channel.
The fluorescence intensity of PE-iFluor® 594 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.