PE-iFluor® 594 Tandem
Product key features
- High Brightness & Photostability - Delivers strong, stable fluorescence (Ex/Em: 565/606 nm), enabling sensitive detection of low-abundance targets.
- Optimized for Flow Cytometers - Efficient excitation with 488/561 nm lasers; compatible with 610/20 nm filters for streamlined multicolor panel design.
- Enhanced Signal Resolution - Higher quantum yield and lower background than PE-Texas Red, improving signal-to-noise ratios and reducing spectral spillover.
Product description
PE-iFluor® 594 is a high-performance tandem fluorophore optimized for flow cytometry, immunophenotyping, and fluorescence-activated cell sorting (FACS). Upon covalent attachment to antibodies or proteins via maleimide-activated crosslinkers such as SMCC, it enables precise detection of cellular markers in complex, multicolor panels.
This tandem dye combines R-phycoerythrin (PE) as the donor and iFluor® 594 as the acceptor, yielding a bright, photostable emission profile with excitation and emission maxima at 565 nm and 606 nm, respectively. PE-iFluor® 594 is efficiently excited by both 488 nm and 561 nm laser lines and is compatible with standard 610/20 nm bandpass filters, facilitating integration into conventional cytometry systems.
Compared to traditional PE-Texas Red conjugates, PE-iFluor® 594 offers enhanced fluorescence intensity, improved quantum yield, and lower background, resulting in superior signal-to-noise ratios and reduced spectral overlap—critical parameters for high-dimensional cytometry. Its robust photostability ensures consistent performance during extended acquisition, enabling accurate detection of low-abundance targets and rare cell populations.
For added convenience, PE-iFluor® 594 is also available in a ReadiUse® preactivated format. Pre-functionalized with Buccutite® FOL chemistry, it enables efficient, thiol-free conjugation. In this system, the target antibody or protein is first modified with Buccutite® MTA, then reacted with the FOL-activated PE-iFluor® 594, producing stable, bioactive conjugates in a rapid, aqueous workflow, ready for immediate use in flow cytometry applications.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
PE-iFluor® 597 Tandem | 565 | 612 | - |
PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
PE-iFluor® 660 Tandem | 565 | 695 | 1960000 |
PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
PE-iFluor® 720 Tandem | 565 | 750 | 1960000 |
PE-iFluor® 740 Tandem | 565 | 767 | 1960000 |
PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
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References
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