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PE-iFluor® 594 Tandem
Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor® 594 or iFluor® 750). PE-iFluor® 594 is a superior replacement to the commonly used PE-Texas Red tandem with significantly improved FRET efficiency and signal/background ratio. Its primary absorption peak is at 565 nm with emission peak around 610 nm. AAT Bioquest also offers a unique preactivated PE-iFluor® 594 to facilitate the PE- iFluor® 594 tandem conjugation to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE- iFluor® 594 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE tandems are conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| PE-iFluor® 700 Tandem | 565 | 708 | 1960000 |
| PE-iFluor® 610 Tandem | 565 | 625 | 1960000 |
| PE-iFluor® 647 Tandem | 565 | 666 | 1960000 |
| PE-iFluor® 750 Tandem | 565 | 778 | 1960000 |
| PE-iFluor® 710 Tandem | 565 | 747 | 1960000 |
| PE-iFluor® 660 Tandem | 565 | 695 | 1960000 |
| PE-iFluor® 780 Tandem | 566 | 575 | 1960000 |
| PE-iFluor® 597 Tandem | 565 | 612 | - |
| PE-iFluor® 740 Tandem | 565 | 767 | 1960000 |
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References
View all 8 references: Citation Explorer
Orange juice and its major polyphenol hesperidin consumption do not induce immunomodulation in healthy well-nourished humans.
Authors: Perche, Olivier and Vergnaud-Gauduchon, Juliette and Morand, Christine and Dubray, Claude and Mazur, Andrzej and Vasson, Marie-Paule
Journal: Clinical nutrition (Edinburgh, Scotland) (2014): 130-5
Authors: Perche, Olivier and Vergnaud-Gauduchon, Juliette and Morand, Christine and Dubray, Claude and Mazur, Andrzej and Vasson, Marie-Paule
Journal: Clinical nutrition (Edinburgh, Scotland) (2014): 130-5
Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity.
Authors: Fromm, Jonathan R and Thomas, Anju and Wood, Brent L
Journal: American journal of clinical pathology (2009): 322-32
Authors: Fromm, Jonathan R and Thomas, Anju and Wood, Brent L
Journal: American journal of clinical pathology (2009): 322-32
Optimization of three- and four-color multiparameter DNA analysis in lymphoma specimens.
Authors: Plander, M and Brockhoff, G and Barlage, S and Schwarz, S and Rothe, G and Knuechel, R
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2003): 66-74
Authors: Plander, M and Brockhoff, G and Barlage, S and Schwarz, S and Rothe, G and Knuechel, R
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2003): 66-74
Multiparameter cytokine-specific affinity matrix assay for the determination of frequencies and phenotype of antigen-reactive T cells.
Authors: Mathioudakis, George and Coder, David and Fefer, Alexander
Journal: Journal of immunological methods (2002): 37-42
Authors: Mathioudakis, George and Coder, David and Fefer, Alexander
Journal: Journal of immunological methods (2002): 37-42
Conjugation of fluorochromes to monoclonal antibodies.
Authors: Holmes, K L and Lantz, L M and Russ, W
Journal: Current protocols in cytometry (2001): Unit 4.2
Authors: Holmes, K L and Lantz, L M and Russ, W
Journal: Current protocols in cytometry (2001): Unit 4.2
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