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OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor
Product key features
- Readily used for live cell imaging
- Provide high selectivity for mitochondrial lipid peroxidation
- Compatible with the common Cy3/TRITC filter set
- Multiplex with other dyes (e.g., DAPI or GFP)
Product description
A wide range of diseases is considered to result from mitochondrial oxidative damage that is associated with the lipid peroxidation of mitochondrial inner membranes. There are no specific methods to assess mitochondrial lipid peroxidation in live cells. To address this unmet need, AAT Bioquest has developed the OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor, a fluorescent mitochondria-targeted probe that detects lipid peroxidation in live cells. OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor enters cells rapidly, and selectively accumulates in mitochondria. It has a high specificity for the detection of mitochondrial lipid peroxidation. Mitochondrial lipid peroxidation results in a great change in fluorescence at 590 nm, which can conveniently be monitored by fluorimetry, fluorescence microscopy or a fluorescence microplate reader.
Example protocol
AT A GLANCE
Before initial use, thaw OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor at room temperature and briefly centrifuge to collect the dried pellet.
Prepare and treat cells as needed in growth medium
Stain cells with OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor working solution
Incubate samples at 37 °C in a 5% CO₂ incubator for 30–60 minutes
Monitor fluorescence intensity with Cy3 filter
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Protect from light and avoid repeated freeze-thaw cycles
Prepare 2 to 5 mM stock solution in DMSO. For example, add 20 μL of DMSO into one vial to create a 5 mM OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor stock solution.
Note: Prepare single-use aliquots of the stock solution and store at ≤ -20°C. Protect from light and avoid repeated freeze-thaw cycles.
PREPARATION OF WORKING SOLUTION
Prepare a 500 to 1000 nM OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor working solution. For example, add 2 μL of 5 mM OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor stock solution to 10 mL of cell culture medium or HHBS buffer (AAT cat# 20011).
Note: Protect the working solution from light by covering it with foil or placing it in the dark.
Note: For best results, use the solution within 2 hours of its preparation.
Note: 10 mL of working solution is enough for 100 tests.
SAMPLE EXPERIMENTAL PROTOCOL
Plate cells as needed in a 96-well black wall, clear bottom plate.
Add treatment to induce lipid peroxidation.
Add 100 µL of OxiVision™ Red Mitochondrial Lipid Peroxidation Sensor working solution to cells.
Incubate cells at 37 ºC in a 5% CO2 incubator for 30–60 minutes, protected from light.
Note: The optimal concentration and incubation time may vary by cell line; we recommend testing with different concentrations.
Optional: Remove the dye working solution and wash cells twice with HHBS buffer if background fluorescence is observed.
Add HHBS buffer and analyze the cells using a fluorescence microscope with a Cy3 filter set.
References
Authors: Huan, He and Lyamzaev, Konstantin G and Panteleeva, Alisa A and Chernyak, Boris V
Journal: Frontiers in cell and developmental biology (2024): 1452824
Authors: Hai, Yongrui and Fan, Renming and Zhao, Ting and Lin, Ruizhuo and Zhuang, Junyan and Deng, Aohua and Meng, Shanshui and Hou, Zhuang and Wei, Gaofei
Journal: Pharmacological research (2024): 107115
Authors: Zhong, Gaolong and Wang, MengRan and Sun, Bingxia and Ma, Feiyang and Yu, Wenlan and Hu, Lianmei and Liao, Jianzhao and Tang, Zhaoxin
Journal: Journal of agricultural and food chemistry (2024): 15948-15958
Authors: Fan, Renming and Deng, Aohua and Lin, Ruizhuo and Zhang, Shuo and Cheng, Caiyan and Zhuang, Junyan and Hai, Yongrui and Zhao, Minggao and Yang, Le and Wei, Gaofei
Journal: MedComm (2024): e570
Authors: Lyamzaev, Konstantin G and Huan, He and Panteleeva, Alisa A and Simonyan, Ruben A and Avetisyan, Armine V and Chernyak, Boris V
Journal: Biomolecules (2024)
Safety data sheet