Nuclear Red™ DCS1 *5 mM DMSO Solution*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 671.42 |
Solvent | DMSO |
Excitation (nm) | 631 |
Emission (nm) | 651 |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Nuclear Blue™ DCS1 *5 mM DMSO Solution* |
Nuclear Green™ DCS1 *5 mM DMSO Solution* |
Nuclear Orange™ DCS1 *5 mM DMSO Solution* |
Overview | SDSProtocol |
Molecular weight 671.42 | Excitation (nm) 631 | Emission (nm) 651 |
Platform
Fluorescence microscope
Excitation | Cy5 Filter |
Emission | Cy5 Filter |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Ex/Em = 631/651 nm (bound to DNA)
SAMPLE EXPERIMENTAL PROTOCOL
Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.
Add Nuclear Red™ DCS1 (2 to10 µM) into the fixed, dead or apoptotic cells (either suspension or adherent) and incubate the cells for 15 to 60 minutes.
Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.
Optional: Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.
Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 148.938 µL | 744.69 µL | 1.489 mL | 7.447 mL | 14.894 mL |
5 mM | 29.788 µL | 148.938 µL | 297.876 µL | 1.489 mL | 2.979 mL |
10 mM | 14.894 µL | 74.469 µL | 148.938 µL | 744.69 µL | 1.489 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
Nuclear Blue™ DCS1 *5 mM DMSO Solution* | 348 | 469 |
Nuclear Green™ DCS1 *5 mM DMSO Solution* | 503 | 527 |
Nuclear Orange™ DCS1 *5 mM DMSO Solution* | 514 | 556 |
Images
Citations
Authors: Su, Yu-Fu and Shen, Po-Chien and Huang, Wen-Yen and Hung, Yi-Jen and Huang, Tsai-Wang and Lin, Che-Yi and Shieh, Yi-Shing
Journal: Journal of Dental Sciences (2023)
Authors: Lin, Li-Fan and Jhao, Yun-Ting and Chiu, Chuang-Hsin and Sun, Lu-Han and Chou, Ta-Kai and Shiue, Chyng-Yann and Cheng, Cheng-Yi and Ma, Kuo-Hsing
Journal: Pharmaceuticals (2022): 109
Authors: Hsiao, Chen-Yuan and Chen, Tien-Hua and Chu, Tzu-Hui and Ting, Yen-Nien and Tsai, Pei-Jiun and Shyu, Jia-Fwu
Journal: Frontiers in Endocrinology (2020): 613
Authors: Zheng, Cai-Mei and Hsu, Yung-Ho and Wu, Chia-Chao and Lu, Chien-Lin and Liu, Wen-Chih and Zheng, Jing-Quan and Lin, Yuh-Feng and Chiu, Hui-Wen and Chang, Tian-Jong and Shyu, Jia-Fwu and others,
Journal: International journal of molecular sciences (2019): 2800
Authors: Sima, Ni and Li, Rong and Huang, Wei and Xu, Miao and Beers, Jeanette and Zou, Jizhong and Titus, Steven and Ottinger, Elizabeth A and Marugan, Juan J and Xie, Xing and others, undefined
Journal: Orphanet journal of rare diseases (2018): 54
Authors: Egele, Kerstin and Samaddar, Shayak and Schneider, Nina and Thompson, David H and Wenz, Gerhard
Journal: Journal of Materials Chemistry B (2018)
Authors: Dai, Sheng and Dulcey, Andr{\'e}s E and Hu, Xin and Wassif, Christopher A and Porter, Forbes D and Austin, Christopher P and Ory, Daniel S and Marugan, Juan and Zheng, Wei
Journal: Autophagy (2017): 1435--1451
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
References
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Journal: Am J Clin Pathol (2008): 706
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Journal: Cytometry A (2008): 555
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759
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Journal: J Comput Biol (2007): 84
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Journal: Nat Protoc (2007): 269
Application notes
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Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?