Nuclear Green™ DCS1 *5 mM DMSO Solution*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | DMSO |
Excitation (nm) | 503 |
Emission (nm) | 527 |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Nuclear Blue™ DCS1 *5 mM DMSO Solution* |
Nuclear Orange™ DCS1 *5 mM DMSO Solution* |
Nuclear Red™ DCS1 *5 mM DMSO Solution* |
Overview | SDSProtocol |
Excitation (nm) 503 | Emission (nm) 527 |
Platform
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Ex/Em = 515/530 nm (bound to DNA)
SAMPLE EXPERIMENTAL PROTOCOL
Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.
Add 2 to 10 µM of Nuclear Green™ DCS1 to fixed, dead, or apoptotic cells (whether in suspension or adherent) and incubate for 15 to 60 minutes.
Note: In order to determine the optimal concentration that yields the desired result, it is advisable to test a wide range of dye concentrations in initial experiments.
Optional: Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.
Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set.
Product Family
Name | Excitation (nm) | Emission (nm) |
Nuclear Blue™ DCS1 *5 mM DMSO Solution* | 348 | 469 |
Nuclear Orange™ DCS1 *5 mM DMSO Solution* | 514 | 556 |
Nuclear Red™ DCS1 *5 mM DMSO Solution* | 631 | 651 |
Images
Citations
Authors: Joaqu{\'\i}n-Ovalle, Freisa M and Guihurt, Grace and Barcelo-Bovea, Vanessa and Hani-Saba, Andraous and Fontanet-G{\'o}mez, Nicole C and Ramirez-Paz, Josell and Kashino, Yasuhiro and Torres-Martinez, Zally and Doble-Cacho, Katerina and Delinois, Louis J and others,
Journal: BioTech (2022): 9
Authors: Le, Trieu Phuong Hai
Journal: (2021)
Authors: Le, Trieu and Le, Son C and Zhang, Yang and Liang, Pengfei and Yang, Huanghe
Journal: Journal of Biological Chemistry (2020): 12537--12544
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
References
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759
Authors: Johnson JD, Dennull RA, Gerena L, Lopez-Sanchez M, Roncal NE, Waters NC.
Journal: Antimicrob Agents Chemother (2007): 1926
Authors: Patel A, Noble RT, Steele JA, Schwalbach MS, Hewson I, Fuhrman JA.
Journal: Nat Protoc (2007): 269
Application notes
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Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
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