Nuclear Blue™ DCS1 *5 mM DMSO Solution*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | N/A |
Solvent | DMSO |
Excitation (nm) | 348 |
Emission (nm) | 469 |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Nuclear Green™ DCS1 *5 mM DMSO Solution* |
Nuclear Orange™ DCS1 *5 mM DMSO Solution* |
Nuclear Red™ DCS1 *5 mM DMSO Solution* |
Overview | SDSProtocol |
Molecular weight N/A | Excitation (nm) 348 | Emission (nm) 469 |
Platform
Fluorescence microscope
Excitation | DAPI Filter |
Emission | DAPI Filter |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Ex/Em = 355/458 nm (bound to DNA)
SAMPLE EXPERIMENTAL PROTOCOL
Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.
Add Nuclear Blue™ DCS1 (2 to10 µM) into the fixed, dead or apoptotic cells (either suspension or adherent) and incubate the cells for 15 to 60 minutes.
Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.
Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.
Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set.
Product Family
Name | Excitation (nm) | Emission (nm) |
Nuclear Green™ DCS1 *5 mM DMSO Solution* | 503 | 527 |
Nuclear Orange™ DCS1 *5 mM DMSO Solution* | 514 | 556 |
Nuclear Red™ DCS1 *5 mM DMSO Solution* | 631 | 651 |
Images
Citations
Authors: Wan, Zhengpeng and Floryan, Marie A and Coughlin, Mark F and Zhang, Shun and Zhong, Amy X and Shelton, Sarah E and Wang, Xun and Xu, Chenguang and Barbie, David A and Kamm, Roger D
Journal: Advanced Healthcare Materials (2022): 2201784
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
References
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84
Authors: Swerts K, Van Roy N, Benoit Y, Laureys G, Philippe J.
Journal: Clin Chim Acta (2007): 154
Application notes
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FAQ
Are there any alternatives to BrdU (Bromodeoxyuridine)?
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