NBD-PE [N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt]

• Environment-Sensitive Fluorophore: NBD head group enables fluorescence changes based on membrane environment.
• Optimal Spectral Properties: Ex/Em maxima at ~467/539 nm.
• Membrane Integration: Efficiently incorporates into lipid bilayers for dynamic studies.
• Versatile Applications: Ideal for studying membrane dynamics, fusion, and lipid interactions.

NBD-PE (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) is a fluorescent phospholipid commonly used in cell biology research for studying membrane dynamics, including membrane fluidity and trafficking. Its ability to integrate seamlessly into cellular membranes and fluoresce upon excitation makes it a valuable tool for investigating the physical and functional properties of lipid bilayers.

This phospholipid is modified at its head group with the environment-sensitive fluorophore NBD, which has excitation and emission maxima at approximately 467 nm and 539 nm, respectively. When paired with complementary dyes such as Rhodamine-DHPE (#60026) or Texas Red®-DHPE (#60027), NBD-PE enables fluorescence resonance energy transfer (FRET)-based assays for studying membrane fusion events. Furthermore, it has been widely applied in fluorescence recovery after photobleaching (FRAP) experiments to quantitatively assess membrane dynamics and lipid mobility.