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NBD-F [4-Fluoro-7-nitrobenzofurazan] *CAS 29270-56-2*
NBD-F has properties and applications similar to those of NBD-Cl. Upon reacting with amines and thiol compounds it generates the same fluorescent adducts as those of NBD-Cl. Compared with NBD-Cl, NBD-F is much more reactive, and should be more carefully stored. For example, the reaction of NBD fluoride with glycine is reported to be 500 times faster than the reaction of NBD chloride with glycine. Both NBD chloride and NBD fluoride are extensively used as derivatization reagents for chromatographic analysis of amino acids and other low molecular weight amines.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-F [4-Fluoro-7-nitrobenzofurazan] *CAS 29270-56-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 546.15 µL | 2.731 mL | 5.461 mL | 27.307 mL | 54.615 mL |
| 5 mM | 109.23 µL | 546.15 µL | 1.092 mL | 5.461 mL | 10.923 mL |
| 10 mM | 54.615 µL | 273.075 µL | 546.15 µL | 2.731 mL | 5.461 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| NBD-Cl [4-Chloro-7-nitrobenzofurazan] *CAS 10199-89-0* | 467 | 539 | 22000 |
| NBD-PE [N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt] | 467 | 539 | 22000 |
Citations
View all 1 citations: Citation Explorer
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
References
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Journal: Embo J (2006): 4728
Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent
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Authors: Higashi Y, Nakamura S, Matsumura H, Fujii Y.
Journal: Biomed Chromatogr (2006): 423
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