mFluor™ Violet 540 Anti-human CD3 Antibody *UCHT1*

The UCHT1 monoclonal antibody binds to human CD3e, a 20 kD member of the Ig superfamily commonly found on the surface of tregs, thymocytes, nkt cells and thymocytes (differentiation dependent)s. In many organisms, CD3 enhances interleukin-4 production, is an inhibitor of gene expression and negatively regulates smoothened signaling pathway. Also, it plays a role in essential cellular pathways, for instance, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 537/65 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Page updated on April 25, 2025