mFluor™ Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*
mFluor™ Violet 510 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ Violet 510 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ Violet 510 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Violet 510 is optimally excited by the violet laser and emits bright green fluorescence maximally at 505 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-rabbit IgG whole antibodies have been cross-adsorbed against human, horse, rabbit, and bovine IgG.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Violet 510 goat anti-mouse IgG (H+L) | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
mFluor™ Violet 510 goat anti-mouse IgG (H+L) *Cross-Absorbed* | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
References
View all 4 references: Citation Explorer
FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
The use of fluorescent target arrays for assessment of T cell responses in vivo.
Authors: Quah, Benjamin J C and Wijesundara, Danushka K and Ranasinghe, Charani and Parish, Christopher R
Journal: Journal of visualized experiments : JoVE (2014): e51627
Authors: Quah, Benjamin J C and Wijesundara, Danushka K and Ranasinghe, Charani and Parish, Christopher R
Journal: Journal of visualized experiments : JoVE (2014): e51627
Use of a new violet-excitable AmCyan variant as a label in cell analysis.
Authors: Guryev, Oleg and Jaimes, Maria C and Edinger, Mark G and Matvienko, Marta and Abrams, Barny and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2012): 627-34
Authors: Guryev, Oleg and Jaimes, Maria C and Edinger, Mark G and Matvienko, Marta and Abrams, Barny and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2012): 627-34
Quantum dots in flow cytometry.
Authors: Abrams, Barnaby and Dubrovsky, Tim
Journal: Methods in molecular biology (Clifton, N.J.) (2007): 185-203
Authors: Abrams, Barnaby and Dubrovsky, Tim
Journal: Methods in molecular biology (Clifton, N.J.) (2007): 185-203
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