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mFluor™ Violet 510 goat anti-mouse IgG (H+L)

mFluor™ Violet 510 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ Violet 510 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the exceptional contrast provided by mFluor™ Violet 510 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Violet 510 is optimally excited by the violet laser and emits bright green fluorescence maximally at 505 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ Violet 510 goat anti-rabbit IgG (H+L)4125052500010.8610.4640.366
mFluor™ Violet 510 goat anti-rabbit IgG (H+L) *Cross-Absorbed*4125052500010.8610.4640.366

References

View all 5 references: Citation Explorer
New violet-excitable reagents for multicolor flow applications.
Authors: Abrams, Barny and Diwu, Zhenjun and Guryev, Oleg and Suni, Maria and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2013): 752-62
Use of a new violet-excitable AmCyan variant as a label in cell analysis.
Authors: Guryev, Oleg and Jaimes, Maria C and Edinger, Mark G and Matvienko, Marta and Abrams, Barny and Dubrovsky, Tim
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2012): 627-34
The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86
Improved compensation of the fluorochrome AmCyan using cellular controls.
Authors: DiGiuseppe, Joseph A and Cardinali, Jolene
Journal: Cytometry. Part B, Clinical cytometry (2011): 191-4
Quantum dots in flow cytometry.
Authors: Abrams, Barnaby and Dubrovsky, Tim
Journal: Methods in molecular biology (Clifton, N.J.) (2007): 185-203
Page updated on December 17, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
4905049051
Quantity
Add to cart

Additional ordering information

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Physical properties

Molecular weight

~150 kDa

Solvent

Water

Spectral properties

Absorbance (nm)

410

Correction Factor (260 nm)

0.464

Correction Factor (280 nm)

0.366

Extinction coefficient (cm -1 M -1)

250001

Excitation (nm)

412

Emission (nm)

505

Quantum yield

0.861

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501