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mFluor™ UV 460 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate contains mFluor™ UV 460 fluorophore that is well excited by the common 350 nm laser. It has a similar spectrum to BD's BV480, and demonstrates excellent avidin binding and strong blue/green fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ UV 460 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ UV 375 Biotin Conjugate | 351 | 387 | 300001 | 0.941 | 0.099 | 0.138 |
References
View all 14 references: Citation Explorer
Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.
Authors: Wan, Zhenzhen and Gong, Fangfang and Zhang, Mimi and He, Leiliang and Wang, Yilin and Yu, Songcheng and Liu, Jie and Wu, Yuming and Liu, Li'e and Wu, Yongjun and Qu, Lingbo and Sun, Jiaqi and Yu, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120020
Authors: Wan, Zhenzhen and Gong, Fangfang and Zhang, Mimi and He, Leiliang and Wang, Yilin and Yu, Songcheng and Liu, Jie and Wu, Yuming and Liu, Li'e and Wu, Yongjun and Qu, Lingbo and Sun, Jiaqi and Yu, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120020
Temperature-Responsive Hydrogel-Coated Gold Nanoshells.
Authors: Park, Hye Hun and Srisombat, La-Ongnuan and Jamison, Andrew C and Liu, Tingting and Marquez, Maria D and Park, Hansoo and Lee, Sungbae and Lee, Tai-Chou and Lee, T Randall
Journal: Gels (Basel, Switzerland) (2018)
Authors: Park, Hye Hun and Srisombat, La-Ongnuan and Jamison, Andrew C and Liu, Tingting and Marquez, Maria D and Park, Hansoo and Lee, Sungbae and Lee, Tai-Chou and Lee, T Randall
Journal: Gels (Basel, Switzerland) (2018)
Genetic engineering, expression, and activity of a chimeric monoclonal antibody-avidin fusion protein for receptor-mediated delivery of biotinylated drugs in humans.
Authors: Boado, Ruben J and Zhang, Yufeng and Zhang, Yun and Xia, Chun-fang and Wang, Yuntao and Pardridge, William M
Journal: Bioconjugate chemistry (2008): 731-9
Authors: Boado, Ruben J and Zhang, Yufeng and Zhang, Yun and Xia, Chun-fang and Wang, Yuntao and Pardridge, William M
Journal: Bioconjugate chemistry (2008): 731-9
Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization.
Authors: Sun, Younguk and Seo, Moo Seok and Kim, Jae Hyun and Kim, Yun Jae and Kim, Gun A and Lee, Jong Il and Lee, Jung-Hyun and Kwon, Suk-Tae
Journal: Environmental microbiology (2008): 3212-24
Authors: Sun, Younguk and Seo, Moo Seok and Kim, Jae Hyun and Kim, Yun Jae and Kim, Gun A and Lee, Jong Il and Lee, Jung-Hyun and Kwon, Suk-Tae
Journal: Environmental microbiology (2008): 3212-24
Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys.
Authors: Van Loock, Marnix and Verminnen, Kristel and Messmer, Trudy O and Volckaert, Guido and Goddeeris, Bruno M and Vanrompay, Daisy
Journal: BMC infectious diseases (2005): 76
Authors: Van Loock, Marnix and Verminnen, Kristel and Messmer, Trudy O and Volckaert, Guido and Goddeeris, Bruno M and Vanrompay, Daisy
Journal: BMC infectious diseases (2005): 76
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