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mFluor™ UV 375 Biotin Conjugate

mFluor™ UV 375 Biotin Conjugate contains mFluor™ UV 375 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong UV fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ UV 375 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
mFluor™ UV 460 Biotin Conjugate3584561500010.8610.350.134

References

View all 47 references: Citation Explorer
Fluorescence In Situ Hybridization of Small Non-Coding RNAs.
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates.
Authors: Udompholkul, Parima and Baggio, Carlo and Gambini, Luca and Sun, Yu and Zhao, Ming and Hoffman, Robert M and Pellecchia, Maurizio
Journal: Molecules (Basel, Switzerland) (2021)
An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity.
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)
Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation spectroscopy with polystyrene polymer dots.
Authors: Huang, Yuyang and Deng, Liyun and Su, Di and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021)
Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic and oxytocinergic neurons of the rat hypothalamic supraoptic nucleus.
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Page updated on November 20, 2024

Ordering information

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Catalog Number3111
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Additional ordering information

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Physical properties

Molecular weight

954.26

Solvent

DMSO

Spectral properties

Absorbance (nm)

342

Correction Factor (260 nm)

0.099

Correction Factor (280 nm)

0.138

Extinction coefficient (cm -1 M -1)

300001

Excitation (nm)

351

Emission (nm)

387

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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