mFluor™ Red 780 goat anti-rabbit IgG (H+L)

mFluor™ Red 780 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ Red 780 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the contrast provided by mFluor™ Red 780 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Red 780 is optimally excited by the red laser and emits NIR fluorescence maximally at 767 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
49350	200 ug	Price
49351	1 mg	Price

References

View all 9 references: Citation Explorer

Tandem dyes: Stability in cocktails and compensation considerations.

Authors:

Johansson, Ulrika and Macey, Marion

Journal:

Cytometry. Part B, Clinical cytometry (2014): 164-74

Tandem Dyes: Stability in cocktails and compensation considerations.

Authors:

Johansson, Ulrika and Macey, Marion

Journal:

Cytometry. Part B, Clinical cytometry (2013)

Flow cytometry and the stability of phycoerythrin-tandem dye conjugates.

Authors:

Hulspas, Ruud and Dombkowski, David and Preffer, Frederic and Douglas, Derick and Kildew-Shah, Brian and Gilbert, John

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 966-72

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.

Authors:

Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90

Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets.

Authors:

Mittag, Anja and Lenz, Dominik and Gerstner, Andreas O H and Sack, Ulrich and Steinbrecher, Michael and Koksch, Mario and Raffael, Alexander and Bocsi, Jozsef and Tárnok, Attila

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 103-15

Physical properties

Molecular weight	~150 kDa
Solvent	Water

Spectral properties

Absorbance (nm)	630
Correction factor (260 nm)	0.101
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	629
Emission (nm)	767

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 15, 2025