mFluor™ Red 780 goat anti-rabbit IgG (H+L)
mFluor™ Red 780 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ Red 780 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the contrast provided by mFluor™ Red 780 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Red 780 is optimally excited by the red laser and emits NIR fluorescence maximally at 767 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications.
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Red 780 goat anti-mouse IgG (H+L) *Cross-Absorbed* | 629 | 767 | 900001 | 0.101 | 0.116 |
mFluor™ Red 780 goat anti-mouse IgG (H+L) | 629 | 767 | 900001 | 0.101 | 0.116 |
References
View all 9 references: Citation Explorer
Tandem dyes: Stability in cocktails and compensation considerations.
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2014): 164-74
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2014): 164-74
Tandem Dyes: Stability in cocktails and compensation considerations.
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2013)
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2013)
Flow cytometry and the stability of phycoerythrin-tandem dye conjugates.
Authors: Hulspas, Ruud and Dombkowski, David and Preffer, Frederic and Douglas, Derick and Kildew-Shah, Brian and Gilbert, John
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 966-72
Authors: Hulspas, Ruud and Dombkowski, David and Preffer, Frederic and Douglas, Derick and Kildew-Shah, Brian and Gilbert, John
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 966-72
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets.
Authors: Mittag, Anja and Lenz, Dominik and Gerstner, Andreas O H and Sack, Ulrich and Steinbrecher, Michael and Koksch, Mario and Raffael, Alexander and Bocsi, Jozsef and Tárnok, Attila
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 103-15
Authors: Mittag, Anja and Lenz, Dominik and Gerstner, Andreas O H and Sack, Ulrich and Steinbrecher, Michael and Koksch, Mario and Raffael, Alexander and Bocsi, Jozsef and Tárnok, Attila
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 103-15
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