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mFluor™ Red 780 goat anti-mouse IgG (H+L) *Cross-Absorbed*

mFluor™ Red 780 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ Red 780 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the contrast provided by mFluor™ Red 780 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Red 780 is optimally excited by the red laser and emits NIR fluorescence maximally at 767 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
mFluor™ Red 780 goat anti-rabbit IgG (H+L) *Cross-Absorbed*6297679000010.1010.116
mFluor™ Red 780 goat anti-rabbit IgG (H+L)6297679000010.1010.116

References

View all 9 references: Citation Explorer
Tandem dyes: Stability in cocktails and compensation considerations.
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2014): 164-74
Tandem Dyes: Stability in cocktails and compensation considerations.
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2013)
Flow cytometry and the stability of phycoerythrin-tandem dye conjugates.
Authors: Hulspas, Ruud and Dombkowski, David and Preffer, Frederic and Douglas, Derick and Kildew-Shah, Brian and Gilbert, John
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 966-72
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets.
Authors: Mittag, Anja and Lenz, Dominik and Gerstner, Andreas O H and Sack, Ulrich and Steinbrecher, Michael and Koksch, Mario and Raffael, Alexander and Bocsi, Jozsef and Tárnok, Attila
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 103-15
Page updated on October 31, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
Quantity
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Physical properties

Molecular weight

~150 kDa

Solvent

Water

Spectral properties

Absorbance (nm)

630

Correction Factor (260 nm)

0.101

Correction Factor (280 nm)

0.116

Extinction coefficient (cm -1 M -1)

900001

Excitation (nm)

629

Emission (nm)

767

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501