mFluor™ Green 620 Anti-human CD16 Antibody 3G8

Name: mFluor™ Green 620 Anti-human CD16 Antibody *3G8*
Brand: AAT Bioquest
SKU: 101610U0
Availability: InStock

The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils. CD16 is a member of key cellular pathways, namely, the Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like IgG Fc. CD16 is a very popular antibody target, with over 25000 publications in the last decade. CD16a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Green 620 (ex/em = 525/623 nm). It is compatible with the 532 nm laser and 610/20 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Spectrum

Open in Advanced Spectrum Viewer

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd16a antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

Page updated on December 17, 2024

Ordering information

Price
Unit size	100 tests 500 tests
Catalog Number	101610U0101610U1
Quantity

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

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Antibody properties

Other names	FCRIIIA
Clone	3G8
Host	mouse
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)	525
Correction Factor (260 nm)	0.895
Correction Factor (280 nm)	0.569
Extinction coefficient (cm ^-1 M ^-1)	50000¹
Excitation (nm)	525
Emission (nm)	623