JC-10 *Superior alternative to JC-1*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 583.34 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 508 |
Emission (nm) | 524 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Mitochondria, Mitochondrial Membrane Potential
Molecular weight 583.34 | Excitation (nm) 508 | Emission (nm) 524 |
Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be an alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers, so that green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its potential use in flow cytometry, it can also be used in fluorescence imaging. We have developed a protocol to use JC-10 in fluorescence microplate platform. In some cell lines JC-10 has even superior performance to JC-1. Interestingly the performance of JC-10 is quite cell line-dependent. Our JC-10 is conveniently provided in DMSO solution at ~3 mM concentration (2 mg/mL).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of JC-10 *Superior alternative to JC-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 171.427 µL | 857.133 µL | 1.714 mL | 8.571 mL | 17.143 mL |
5 mM | 34.285 µL | 171.427 µL | 342.853 µL | 1.714 mL | 3.429 mL |
10 mM | 17.143 µL | 85.713 µL | 171.427 µL | 857.133 µL | 1.714 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
Images
![Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fjc-10-superior-alternative-to-jc-1%2Ffigure-for-jc-10-superior-alternative-to-jc-1_zwaTq.jpg&w=3840&q=75)
Figure 1. Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Citations
View all 76 citations: Citation Explorer
Green synthesis and characterization of AgNPs, liposomal loaded AgNPs and ZnPcS4 photosensitizer for enhanced photodynamic therapy effects in MCF-7 breast cancer cells
Authors: Chota, Alexander and Abrahamse, Heidi and George, Blassan P
Journal: Photodiagnosis and Photodynamic Therapy (2024): 104252
Authors: Chota, Alexander and Abrahamse, Heidi and George, Blassan P
Journal: Photodiagnosis and Photodynamic Therapy (2024): 104252
Xiaohuang Lin1, Jian Zhang1, 2, Zekai Wu1, Yuan Shi3, Mengting Chen1, Maodong Li4, Hong Hu2, Kun Tian1, Xiaoqi Lv5, Chutao Li1, Yang Liu1, Xinyue Gao1, Qiaomei Yang6, Kunqi Chen1* and An Zhu1
Authors: Lin, X and Zhang, J and Wu, Z and Shi, Y and Chen, M and Li, M and Hu, H and Tian, K and Lv, X and Li, C and others,
Journal: (2024)
Authors: Lin, X and Zhang, J and Wu, Z and Shi, Y and Chen, M and Li, M and Hu, H and Tian, K and Lv, X and Li, C and others,
Journal: (2024)
Chlorogenic Acid as a Potential Therapeutic Agent for Cholangiocarcinoma
Authors: Liang, Jiabao and Wen, Tong and Zhang, Xiaojian and Luo, Xiaoling
Journal: Pharmaceuticals (2024): 794
Authors: Liang, Jiabao and Wen, Tong and Zhang, Xiaojian and Luo, Xiaoling
Journal: Pharmaceuticals (2024): 794
Effect of autologous platelet-rich plasma on the fertility and quality of cryopreserved buffalo bull semen: a comparative study using OptiXcell{\textregistered} and tris egg yolk extenders
Authors: Salama, Maha S and Ashour, Mohey A and Taher, Ehab S and Rashed, Fatema and Ibrahim, Ibrahim M and El-Nablaway, Mohammad and Ibrahim, Ateya Megahed and Mihaela, Ostan and Olga, Rada and Mohammed, Nourelhuda A and others,
Journal: BMC Veterinary Research (2024): 1--10
Authors: Salama, Maha S and Ashour, Mohey A and Taher, Ehab S and Rashed, Fatema and Ibrahim, Ibrahim M and El-Nablaway, Mohammad and Ibrahim, Ateya Megahed and Mihaela, Ostan and Olga, Rada and Mohammed, Nourelhuda A and others,
Journal: BMC Veterinary Research (2024): 1--10
The activation of AMPK/PGC-1$\alpha$/GLUT4 signaling pathway through early exercise improves mitochondrial function and mitigates ischemic brain damage
Authors: Wu, Xinyue and Li, Chen and Ke, Changkai and Huang, Chuan and Pan, Bingchen and Wan, Chunxiao
Journal: NeuroReport (2024): 648--656
Authors: Wu, Xinyue and Li, Chen and Ke, Changkai and Huang, Chuan and Pan, Bingchen and Wan, Chunxiao
Journal: NeuroReport (2024): 648--656
Search for new biologically active compounds: in vitro studies of antitumor and antimicrobial activity of dirhodium (II, II) paddlewheel complexes
Authors: Mitrovi{\'c}, Marina and Djuki{\'c}, Maja B and Vuki{\'c}, Milena and Nikoli{\'c}, Ivana and Radovanovi{\'c}, Marko D and Lukovi{\'c}, Jovan and Filipovi{\'c}, Ignjat P and Mati{\'c}, Sanja and Markovi{\'c}, Tijana and Klisuri{\'c}, Olivera R and others,
Journal: Dalton Transactions (2024)
Authors: Mitrovi{\'c}, Marina and Djuki{\'c}, Maja B and Vuki{\'c}, Milena and Nikoli{\'c}, Ivana and Radovanovi{\'c}, Marko D and Lukovi{\'c}, Jovan and Filipovi{\'c}, Ignjat P and Mati{\'c}, Sanja and Markovi{\'c}, Tijana and Klisuri{\'c}, Olivera R and others,
Journal: Dalton Transactions (2024)
Repurposing of Zoledronic Acid for Treatment of Duchenne Muscular Dystrophy
Authors: Haroon, Alexa Grey
Journal: (2024)
Authors: Haroon, Alexa Grey
Journal: (2024)
Antifungal activity of human antimicrobial peptides targeting apoptosis in Candida auris
Authors: Shaban, Siham and Patel, Mrudula and Ahmad, Aijaz
Journal: Journal of Medical Microbiology (2024): 001835
Authors: Shaban, Siham and Patel, Mrudula and Ahmad, Aijaz
Journal: Journal of Medical Microbiology (2024): 001835
Amorphous CaCO3-bioreactor for tumor microenvironment regulation to reinforce tumor chemoimmunotherapy
Authors: Chen, Weiguo and Lu, Yishuang and Xu, Yize and Chen, Yujiang and Lin, Shuai and He, Xin and Zhang, Chunfeng and Yuan, Chunsu
Journal: Chemical Engineering Journal (2024): 151838
Authors: Chen, Weiguo and Lu, Yishuang and Xu, Yize and Chen, Yujiang and Lin, Shuai and He, Xin and Zhang, Chunfeng and Yuan, Chunsu
Journal: Chemical Engineering Journal (2024): 151838
Chemical diversity and antimelanoma potential of rosemary essential oils: Unveiling mechanistic insights through quantitative proteomics
Authors: Huang, Yeqin and Ding, Mengting and Wang, Di and Li, Hui and Xia, Fei and Bai, Hongtong and Sun, Meiyu and Mo, Meiling and Dong, Yanmei and Shi, Lei
Journal: Industrial Crops and Products (2024): 118652
Authors: Huang, Yeqin and Ding, Mengting and Wang, Di and Li, Hui and Xia, Fei and Bai, Hongtong and Sun, Meiyu and Mo, Meiling and Dong, Yanmei and Shi, Lei
Journal: Industrial Crops and Products (2024): 118652
References
View all 6 references: Citation Explorer
Tissue plasminogen activator regulates Purkinje neuron development and survival
Authors: Jianxue Li, Lili Yu, Xuesong Gu, Yinghua Ma, Renata Pasqualini, Wadih Arap, Evan Y. Snyder, and Richard L. Sidman, undefined
Journal: PNAS (2013): E2410 - E2419
Authors: Jianxue Li, Lili Yu, Xuesong Gu, Yinghua Ma, Renata Pasqualini, Wadih Arap, Evan Y. Snyder, and Richard L. Sidman, undefined
Journal: PNAS (2013): E2410 - E2419
5,6-Dimethylxanthenone-4-acetic Acid (DMXAA) Activates Stimulator of Interferon Gene (STING)-dependent Innate Immune Pathways and Is Regulated by Mitochondrial Membrane Potential
Authors: Prantner D, Perkins DJ, Lai W, Williams MS, Sharma S, Fitzgerald KA, Vogel SN
Journal: J Biol Chem (2012): 39776-88
Authors: Prantner D, Perkins DJ, Lai W, Williams MS, Sharma S, Fitzgerald KA, Vogel SN
Journal: J Biol Chem (2012): 39776-88
Application of a homogenous membrane potential assay to assess mitochondrial function
Authors: Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M
Journal: Physiol Genomics (2012): 495-503
Authors: Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M
Journal: Physiol Genomics (2012): 495-503
Human iPS-derived Cardiomyocytes for Cardiotoxicity Screening
Authors: , undefined
Journal: SBS Molecular Devices (2011)
Authors: , undefined
Journal: SBS Molecular Devices (2011)
iCell® Cardiomyocytes: Assaying Mitochondrial Membrane Potential
Authors: , undefined
Journal: http://www.cellulardynamics.com/products/lit/CDI_appnote_Cardiomyocytes_mitochondrial.pdf
Authors: , undefined
Journal: http://www.cellulardynamics.com/products/lit/CDI_appnote_Cardiomyocytes_mitochondrial.pdf
Analyzing Cellular Apoptosis Through Monitoring Mitochondrial Membrane Potential Changes with JC-10
Authors: Jinfang Liao, Qin Zhao, Xing Han, Zhenjun Diwu
Journal: Biophysical Journal : 2
Authors: Jinfang Liao, Qin Zhao, Xing Han, Zhenjun Diwu
Journal: Biophysical Journal : 2
Application notes
High-Content High-Throughput Assays and iPSC Neuronal Cultures
Midazolam Induces Cellular Apoptosis in Human Cancer Cells
Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Susceptibility of gametes and embryos of the eastern oyster
Midazolam Induces Cellular Apoptosis in Human Cancer Cells
Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Susceptibility of gametes and embryos of the eastern oyster
FAQ
Why is determining cell viability important?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Does propidium iodide stain apoptotic cells?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Can Cat#22666 stain mitochondria in dead cells?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Does propidium iodide stain apoptotic cells?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Can Cat#22666 stain mitochondria in dead cells?
AssayWise
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Comprehensive Guide to Cell Health Assays in Flow Cytometry: A Multidimensional Exploration
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Fluorescent Tools for Studying Mitochondrial Morphology and Function
Comprehensive Guide to Cell Health Assays in Flow Cytometry: A Multidimensional Exploration
Fluorescent Phalloidin: A Practical Stain for Visualizing Actin Filaments
Acetylcholinesterase Quantification