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iFluor® 680 Anti-human CD253 Antibody *2E5*

2E5 is an anti-human monoclonal antibody that recognizes the CD253 antigen. CD253 (sometimes called Apo-2L, TRAIL or TNFSF10) is a 30 kD member of the TNF ligand superfamily that is expressed on the surface of cells such as B cells and macrophages. CD253 is associated with a variety of biologically interesting macromolecules/ligands, for example, apo2. CD253 is a relatively rare antibody target, with fewer than 100 publications in the last decade. Even still, CD253 is essential for immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 680 (ex/em = 684/701 nm).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd253 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on April 22, 2025

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
125300I0125300I1
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesTRAIL; Apo-2L; TNFSF10
Clone2E5
Hostmouse
IsotypeMouse IgG1
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.097

Correction Factor (280 nm)

0.094

Extinction coefficient (cm -1 M -1)

2200001

Excitation (nm)

684

Emission (nm)

701

Quantum yield

0.231

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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