Helixyte™ 14 [Equivalent to SYBR 14]
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 489 |
Emission (nm) | 520 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
Helixyte™ 14 [Equivalent to SYBR 14] *5 mM* |
Related products
Overview | SDSProtocol |
See also: Nucleus
Molecular weight N/A | Excitation (nm) 489 | Emission (nm) 520 |
Helixyte™ 14 has the same chemical structure of SYBR 14 (SYBR is the trademark of ThermoFisher). It is commonly used to monitor the viability of sperms. The proportion of living sperm in semen can be assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI). Helixyte™ 14 is a nucleic acid stain, maximally absorbs at 488 nm and emits at 518 nm when bound to DNA. It stains the nuclei of living sperm bright green. Conversely, PI stains only nonmotile sperm that had lost their membrane integrity. The proportions of living and dead sperm can be determined by first staining with SYBR-14 and PI and then assessing stain uptake by flow cytometry. The proportions of living and dead sperm in mammalian semen can be readily identified through use of dual staining with SYBR-14 and PI and quantified through use of flow cytometry.
Product Family
Name | Excitation (nm) | Emission (nm) |
Helixyte™ Green *20X Aqueous PCR Solution* | 498 | 522 |
Helixyte™ Green *10,000X Aqueous PCR Solution* | 498 | 522 |
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
PBMC Immunophenotyping by Flow Cytometry
Fluorescent Oligonucleotide Labeling Reagents
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
PBMC Immunophenotyping by Flow Cytometry
FAQ
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any cytotoxicity information for your Nuclear LCS dyes?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What is FRET?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any cytotoxicity information for your Nuclear LCS dyes?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What is FRET?