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FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5*
FMOC-Lys(5-FAM)-OH is a building block for in-sequence Lys labeling by FAM. FAM is one of the most common donor dyes for preparing FRET peptides (often paired with TAMRA or TQ2).
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 137.603 µL | 688.013 µL | 1.376 mL | 6.88 mL | 13.76 mL |
| 5 mM | 27.521 µL | 137.603 µL | 275.205 µL | 1.376 mL | 2.752 mL |
| 10 mM | 13.76 µL | 68.801 µL | 137.603 µL | 688.013 µL | 1.376 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| FMOC-Asp(5-FAM)-OH | 493 | 517 | 83000 | 0.32 | 0.178 |
| FMOC-Glu(5-FAM)-OH | 493 | 517 | 83000 | 0.32 | 0.178 |
| FMOC-Lys(5-TAMRA)-OH | 552 | 578 | 90000 | 0.32 | 0.178 |
| FMOC-Lys(5-FITC)-OH *CAS 1095493-05-2* | 491 | 516 | 73000 | - | 0.35 |
Citations
View all 6 citations: Citation Explorer
Single-step purification of a small non-mAb biologic by peptide-ELP-based affinity precipitation
Authors: Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj
Journal: Biotechnology and Bioengineering (2020): 3775--3784
Authors: Mullerpatan, Akshat and Kane, Erin and Ghosh, Ronit and Nascimento, Andr{\'e} and Andersen, Henrik and Cramer, Steven and Karande, Pankaj
Journal: Biotechnology and Bioengineering (2020): 3775--3784
Effect of the hydrophilic-hydrophobic balance of antigen-loaded peptide nanofibers on their cellular uptake, cellular toxicity, and immune stimulatory properties
Authors: Waku, Tomonori and Nishigaki, Saki and Kitagawa, Yuichi and Koeda, Sayaka and Kawabata, Kazufumi and Kunugi, Shigeru and Kobori, Akio and Tanaka, Naoki
Journal: International journal of molecular sciences (2019): 3781
Authors: Waku, Tomonori and Nishigaki, Saki and Kitagawa, Yuichi and Koeda, Sayaka and Kawabata, Kazufumi and Kunugi, Shigeru and Kobori, Akio and Tanaka, Naoki
Journal: International journal of molecular sciences (2019): 3781
Development of Phage Biopanning Strategies to Identify Affinity Peptide Ligands for Kappa Light Chain Fab Fragments
Authors: Nascimento, André and Mullerpatan, Akshat and Azevedo, Ana M and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Biotechnology progress (2019)
Authors: Nascimento, André and Mullerpatan, Akshat and Azevedo, Ana M and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Biotechnology progress (2019)
Purification of Proteins using Peptide-ELP Based Affinity Precipitation
Authors: Mullerpatan, Akshat and Ch, undefined and ra, Divya and Kane, Erin and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Journal of Biotechnology (2019)
Authors: Mullerpatan, Akshat and Ch, undefined and ra, Divya and Kane, Erin and Kar, undefined and e, Pankaj and Cramer, Steven
Journal: Journal of Biotechnology (2019)
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
References
View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
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