FMOC-Asp(TQ2)-OH

FMOC-Asp(TQ2)-OH is a building block for in-sequence Lys labeling by Tide Quencher™ 2 (TQ2). TQ2 is the best quencher that is used to develop a FRET probe with fluorescein or other fluorescent dyes that have the similar fluorescence spectra such as Bodipy FL dyes. It can also be used with rhodamine 6G, 6-TET and 6-JOE derivatives.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Asp(TQ2)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	131.253 µL	656.263 µL	1.313 mL	6.563 mL	13.125 mL
5 mM	26.251 µL	131.253 µL	262.505 µL	1.313 mL	2.625 mL
10 mM	13.125 µL	65.626 µL	131.253 µL	656.263 µL	1.313 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)	Correction Factor (260 nm)
FMOC-Asp(EDANS)-OH CAS 182253-73-2	5900	0.107	-
FMOC-Asp(TF3)-OH	75000¹	0.179	-
FMOC-Asp(DABCYL)-OH	-	0.516	-
FMOC-Asp(TQ3)-OH	22000	0.091	0.085
FMOC-Glu(TQ2)-OH	21000	0.12	0.100
FMOC-Lys(TQ2)-OH	21000	0.12	0.100

Citations

View all 2 citations: Citation Explorer

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196

Page updated on October 28, 2024

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Catalog Number	5206
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Physical properties

Molecular weight	761.89
Solvent	DMF

Spectral properties

Absorbance (nm)	516
Correction Factor (260 nm)	0.100
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	21000

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200