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FMOC-Asp(DABCYL)-OH

A building block for in-sequence Asp labeling by DABCYL.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Asp(DABCYL)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM154.152 µL770.76 µL1.542 mL7.708 mL15.415 mL
5 mM30.83 µL154.152 µL308.304 µL1.542 mL3.083 mL
10 mM15.415 µL77.076 µL154.152 µL770.76 µL1.542 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExtinction coefficient (cm -1 M -1)Correction Factor (280 nm)
FMOC-Asp(EDANS)-OH *CAS 182253-73-2*59000.107
FMOC-Asp(TF3)-OH7500010.179
FMOC-Lys(DABCYL)-OH *CAS 146998-27-8*-0.516
FMOC-Asp(TQ2)-OH210000.12
FMOC-Asp(TQ3)-OH220000.091
FMOC-Glu(DABCYL)-OH-0.516

Citations

View all 2 citations: Citation Explorer
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Page updated on November 20, 2024

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Catalog Number5204
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Physical properties

Molecular weight

648.71

Solvent

DMF

Spectral properties

Absorbance (nm)

454

Correction Factor (280 nm)

0.516

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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