FluoroQuest™ Mounting Medium with DAPI

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Spectrum

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Citations

View all 10 citations: Citation Explorer

Hippo signaling pathway regulates Ebola virus transcription and egress
Authors: Liang, Jingjing and Djurkovic, Marija A and Leavitt, Carson G and Shtanko, Olena and Harty, Ronald N
Journal: Nature Communications (2024): 6953

Zika Virus-Infected Monocyte Exosomes Mediate Cell-to-Cell Viral Transmission
Authors: Mart{\'\i}nez-Rojas, Pedro Pablo and Monroy-Mart{\'\i}nez, Ver{\'o}nica and Agredano-Moreno, Lourdes Teresa and Jim{\'e}nez-Garc{\'\i}a, Luis Felipe and Ruiz-Ordaz, Blanca H
Journal: Cells (2024): 144

Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm
Authors: Kouadio, Jean-Louis and Zheng, Meiying and Aikins, Michael and Duda, David and Duff, Stephen and Chen, Danqi and Zhang, Jun and Milligan, Jason and Taylor, Christina and Mamanella, Patricia and others,
Journal: PloS one (2021): e0260532

Structural and functional characterization of Mpp75Aa1. 1, a putative beta-pore forming protein from Brevibacillus laterosporus active against the western corn rootworm
Authors: Kouadio, Jean-Louis and Duff, Stephen and Aikins, Michael and Zheng, Meiying and Rydel, Timothy and Chen, Danqi and Bretsnyder, Eric and Xia, Chunsheng and Zhang, Jun and Milligan, Jason and others,
Journal: PloS one (2021): e0258052

Acute melatonin administration improves exercise tolerance and the metabolic recovery after exhaustive effort
Authors: Faria, Vin{\'\i}cius Silva and Pejon, Taciane Maria Melges and Gobatto, Claudio Alexandre and de Araujo, Gustavo Gomes and Cornachione, Anabelle Silva and Beck, Wladimir Rafael
Journal: Scientific Reports (2021): 1--11

References

View all 18 references: Citation Explorer

On the mechanism of Trolox as antiblinking and antibleaching reagent
Authors: Cordes T, Vogelsang J, Tinnefeld P.
Journal: J Am Chem Soc (2009): 5018

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification
Authors: Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.
Journal: Biol Res (2007): 29

Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy
Authors: Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.
Journal: J Phys Chem A (2007): 429

The reliability of long-term storage of direct immunofluorescent staining slides at room temperature
Authors: Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.
Journal: J Cutan Pathol (2003): 430

Comparative genomic hybridization technique
Authors: El-Rifai WE, Knuutila S.
Journal: Methods Mol Med (2001): 25

Page updated on December 17, 2024

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Catalog Number	20004
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Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200