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Fluoro-Jade®B
Fluoro-Jade® B is a polyanionic fluorescein derivative which sensitively and specifically binds to degenerating neurons. It has a green fluorescence with excitation peak at 480 nm and emission peak at 525 nm. The filter used for visualizing Fluoro-Jade B is the FITC filter. Fluoro-Jade B can be used on most tissue section types and thicknesses including frozen, vibratomed cryostat or paraffin-embedded sections from 3-50 um. Fluoro-Jade B is faster and more reliable than older methods (e.g. suppressed silver) for the unequivocal qualitative detection and quantitative measurement of both gross and fine scale neuronal degeneration. Fluoro-Jade B will not distinguish between apoptotic and necrotic neurons. Astrocytic labeling is occasionally observed with Fluoro-Jade B, especially in chronic studies. Fluoro-Jade® is a registered trademark of Histo-Chem, Inc.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluoro-Jade®B to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 146.843 µL | 734.214 µL | 1.468 mL | 7.342 mL | 14.684 mL |
| 5 mM | 29.369 µL | 146.843 µL | 293.686 µL | 1.468 mL | 2.937 mL |
| 10 mM | 14.684 µL | 73.421 µL | 146.843 µL | 734.214 µL | 1.468 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Fluoro-Jade®C | 506 | 527 |
Citations
View all 6 citations: Citation Explorer
Critical involvement of lysyl oxidase in seizure-induced neuronal damage through ERK-Alox5-dependent ferroptosis and its therapeutic implications
Authors: Mao, Xiaoyuan and Wang, Xuan and Jin, Mingzhu and Li, Qin and Jia, Jining and Li, Menghuan and Zhou, Honghao and Liu, Zhaoqian and Jin, Weilin and Zhao, Yanli and others,
Journal: Acta Pharmaceutica Sinica B (2022)
Authors: Mao, Xiaoyuan and Wang, Xuan and Jin, Mingzhu and Li, Qin and Jia, Jining and Li, Menghuan and Zhou, Honghao and Liu, Zhaoqian and Jin, Weilin and Zhao, Yanli and others,
Journal: Acta Pharmaceutica Sinica B (2022)
CircTYW1 serves as a sponge for microRNA-380 in accelerating neurological recovery following spinal cord injury via regulating FGF9
Authors: Sun, Yanpeng and Zhou, Yingjie and Shi, Xiangqin and Ma, Xiaoran and Peng, Xiaodong and Xie, Yan and Cao, Xiangyang
Journal: Cell Cycle (2021): 1828--1844
Authors: Sun, Yanpeng and Zhou, Yingjie and Shi, Xiangqin and Ma, Xiaoran and Peng, Xiaodong and Xie, Yan and Cao, Xiangyang
Journal: Cell Cycle (2021): 1828--1844
LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/$\beta$-catenin signaling pathway
Authors: Jiang, Jingjing and Piao, Xuanyu and Hu, Siying and Gao, Jingbo and Bao, Min
Journal: Aging (Albany NY) (2020): 8820
Authors: Jiang, Jingjing and Piao, Xuanyu and Hu, Siying and Gao, Jingbo and Bao, Min
Journal: Aging (Albany NY) (2020): 8820
FTY720 attenuates behavioral deficits in a murine model of systemic lupus erythematosus
Authors: Shi, Dongyan and Tian, Tongguan and Yao, Shu and Cao, Kelei and Zhu, Xingxing and Zhang, Mingshun and Wen, Shuang and Li, Longjun and Shi, Meiqing and Zhou, Hong
Journal: Brain, behavior, and immunity (2018)
Authors: Shi, Dongyan and Tian, Tongguan and Yao, Shu and Cao, Kelei and Zhu, Xingxing and Zhang, Mingshun and Wen, Shuang and Li, Longjun and Shi, Meiqing and Zhou, Hong
Journal: Brain, behavior, and immunity (2018)
Early Minocycline and Late FK506 Treatment Improves Survival and Alleviates Neuroinflammation, Neurodegeneration, and Behavioral Deficits in Prion-Infected Hamsters
Authors: Shah, Syed Zahid Ali and Zhao, Deming and Taglialatela, Giulio and Khan, Sher Hayat and Hussain, Tariq and Dong, Haodi and Lai, Mengyu and Zhou, Xiangmei and Yang, Lifeng
Journal: Neurotherapeutics (2017): 1--21
Authors: Shah, Syed Zahid Ali and Zhao, Deming and Taglialatela, Giulio and Khan, Sher Hayat and Hussain, Tariq and Dong, Haodi and Lai, Mengyu and Zhou, Xiangmei and Yang, Lifeng
Journal: Neurotherapeutics (2017): 1--21
References
View all 38 references: Citation Explorer
Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Gerbil Septum Submitted to Various Durations of Cerebral Ischemia
Authors: Park CW, Lee JC, Ahn JH, Lee DH, Cho GS, Yan BC, Park JH, Kim IH, Lee HY, Won MH, Cho JH.
Journal: Cell Mol Neurobiol. (2013)
Authors: Park CW, Lee JC, Ahn JH, Lee DH, Cho GS, Yan BC, Park JH, Kim IH, Lee HY, Won MH, Cho JH.
Journal: Cell Mol Neurobiol. (2013)
Neuronal damage using fluoro-jade B histofluorescence and gliosis in the striatum after various durations of transient cerebral ischemia in gerbils
Authors: Ohk TG, Yoo KY, Park SM, Shin BN, Kim IH, Park JH, Ahn HC, Lee YJ, Kim MJ, Kim TY, Won MH, Cho JH.
Journal: Neurochem Res (2012): 826
Authors: Ohk TG, Yoo KY, Park SM, Shin BN, Kim IH, Park JH, Ahn HC, Lee YJ, Kim MJ, Kim TY, Won MH, Cho JH.
Journal: Neurochem Res (2012): 826
Neuronal damage in hippocampal subregions induced by various durations of transient cerebral ischemia in gerbils using Fluoro-Jade B histofluorescence
Authors: Yu DK, Yoo KY, Shin BN, Kim IH, Park JH, Lee CH, Choi JH, Cho YJ, Kang IJ, Kim YM, Won MH.
Journal: Brain Res (2012): 50
Authors: Yu DK, Yoo KY, Shin BN, Kim IH, Park JH, Lee CH, Choi JH, Cho YJ, Kang IJ, Kim YM, Won MH.
Journal: Brain Res (2012): 50
One-step labeling of degenerative neurons in unfixed brain tissue samples using Fluoro-Jade C
Authors: Gu Q, Schmued LC, Sarkar S, Paule MG, Raymick B.
Journal: J Neurosci Methods (2012): 40
Authors: Gu Q, Schmued LC, Sarkar S, Paule MG, Raymick B.
Journal: J Neurosci Methods (2012): 40
Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest
Authors: Wang R, Ma WG, Gao GD, Mao QX, Zheng J, Sun LZ, Liu YL.
Journal: Brain Res (2011): 127
Authors: Wang R, Ma WG, Gao GD, Mao QX, Zheng J, Sun LZ, Liu YL.
Journal: Brain Res (2011): 127
Page updated on July 12, 2023
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