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Fluoro-Jade®C
Fluoro-Jade® C, like its predecessors Fluoro-Jade and Fluoro-Jade B, will stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jade dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. Fluoro-Jade® is a registered trademark of Histo-Chem, Inc.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluoro-Jade®C to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 121.507 µL | 607.533 µL | 1.215 mL | 6.075 mL | 12.151 mL |
| 5 mM | 24.301 µL | 121.507 µL | 243.013 µL | 1.215 mL | 2.43 mL |
| 10 mM | 12.151 µL | 60.753 µL | 121.507 µL | 607.533 µL | 1.215 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Fluoro-Jade®B | 494 | 519 |
Citations
View all 8 citations: Citation Explorer
T cell infiltration into the brain triggers pulmonary dysfunction in murine Cryptococcus-associated IRIS
Authors: Kawano, Tasuku and Zhou, Jinyan and Anwar, Shehata and Salah, Haneen and Dayal, Andrea H and Ishikawa, Yuzuki and Boetel, Katelyn and Takahashi, Tomoko and Sharma, Kamal and Inoue, Makoto
Journal: Nature Communications (2023): 3831
Authors: Kawano, Tasuku and Zhou, Jinyan and Anwar, Shehata and Salah, Haneen and Dayal, Andrea H and Ishikawa, Yuzuki and Boetel, Katelyn and Takahashi, Tomoko and Sharma, Kamal and Inoue, Makoto
Journal: Nature Communications (2023): 3831
Cervical Vagus Nerve Stimulation Improves Neurologic Outcome After Cardiac Arrest in Mice by Attenuating Oxidative Stress and Excessive Autophagy
Authors: Duan, Weina and Sun, Qian and Wu, Xiaojing and Xia, Zhongyuan and Warner, David S and Ulloa, Luis and Yang, Wei and Sheng, Huaxin
Journal: Neuromodulation: Technology at the Neural Interface (2022): 414--423
Authors: Duan, Weina and Sun, Qian and Wu, Xiaojing and Xia, Zhongyuan and Warner, David S and Ulloa, Luis and Yang, Wei and Sheng, Huaxin
Journal: Neuromodulation: Technology at the Neural Interface (2022): 414--423
CircTYW1 serves as a sponge for microRNA-380 in accelerating neurological recovery following spinal cord injury via regulating FGF9
Authors: Sun, Yanpeng and Zhou, Yingjie and Shi, Xiangqin and Ma, Xiaoran and Peng, Xiaodong and Xie, Yan and Cao, Xiangyang
Journal: Cell Cycle (2021): 1828--1844
Authors: Sun, Yanpeng and Zhou, Yingjie and Shi, Xiangqin and Ma, Xiaoran and Peng, Xiaodong and Xie, Yan and Cao, Xiangyang
Journal: Cell Cycle (2021): 1828--1844
Determinants of optogenetic cortical spreading depolarizations
Authors: Chung, David Y and Sadeghian, Homa and Qin, Tao and Lule, Sevda and Lee, Hang and Karakaya, Fahri and Goins, Stacy and Oka, Fumiaki and Yaseen, Mohammad A and Houben, Thijs and others,
Journal: Cerebral Cortex (2019): 1150--1161
Authors: Chung, David Y and Sadeghian, Homa and Qin, Tao and Lule, Sevda and Lee, Hang and Karakaya, Fahri and Goins, Stacy and Oka, Fumiaki and Yaseen, Mohammad A and Houben, Thijs and others,
Journal: Cerebral Cortex (2019): 1150--1161
Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway
Authors: Shah, Syed Zahid Ali and Zhao, Deming and Taglialatela, Giulio and Hussain, Tariq and Dong, Haodi and Sabir, Naveed and Mangi, Mazhar Hussain and Wu, Wei and Lai, Mengyu and Zhang, Xixi and others, undefined
Journal: International journal of molecular sciences (2019): 1144
Authors: Shah, Syed Zahid Ali and Zhao, Deming and Taglialatela, Giulio and Hussain, Tariq and Dong, Haodi and Sabir, Naveed and Mangi, Mazhar Hussain and Wu, Wei and Lai, Mengyu and Zhang, Xixi and others, undefined
Journal: International journal of molecular sciences (2019): 1144
References
View all 38 references: Citation Explorer
Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Gerbil Septum Submitted to Various Durations of Cerebral Ischemia
Authors: Park CW, Lee JC, Ahn JH, Lee DH, Cho GS, Yan BC, Park JH, Kim IH, Lee HY, Won MH, Cho JH.
Journal: Cell Mol Neurobiol. (2013)
Authors: Park CW, Lee JC, Ahn JH, Lee DH, Cho GS, Yan BC, Park JH, Kim IH, Lee HY, Won MH, Cho JH.
Journal: Cell Mol Neurobiol. (2013)
Neuronal damage in hippocampal subregions induced by various durations of transient cerebral ischemia in gerbils using Fluoro-Jade B histofluorescence
Authors: Yu DK, Yoo KY, Shin BN, Kim IH, Park JH, Lee CH, Choi JH, Cho YJ, Kang IJ, Kim YM, Won MH.
Journal: Brain Res (2012): 50
Authors: Yu DK, Yoo KY, Shin BN, Kim IH, Park JH, Lee CH, Choi JH, Cho YJ, Kang IJ, Kim YM, Won MH.
Journal: Brain Res (2012): 50
One-step labeling of degenerative neurons in unfixed brain tissue samples using Fluoro-Jade C
Authors: Gu Q, Schmued LC, Sarkar S, Paule MG, Raymick B.
Journal: J Neurosci Methods (2012): 40
Authors: Gu Q, Schmued LC, Sarkar S, Paule MG, Raymick B.
Journal: J Neurosci Methods (2012): 40
Neuronal damage using fluoro-jade B histofluorescence and gliosis in the striatum after various durations of transient cerebral ischemia in gerbils
Authors: Ohk TG, Yoo KY, Park SM, Shin BN, Kim IH, Park JH, Ahn HC, Lee YJ, Kim MJ, Kim TY, Won MH, Cho JH.
Journal: Neurochem Res (2012): 826
Authors: Ohk TG, Yoo KY, Park SM, Shin BN, Kim IH, Park JH, Ahn HC, Lee YJ, Kim MJ, Kim TY, Won MH, Cho JH.
Journal: Neurochem Res (2012): 826
Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest
Authors: Wang R, Ma WG, Gao GD, Mao QX, Zheng J, Sun LZ, Liu YL.
Journal: Brain Res (2011): 127
Authors: Wang R, Ma WG, Gao GD, Mao QX, Zheng J, Sun LZ, Liu YL.
Journal: Brain Res (2011): 127
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