Fluorescein DHPE

Fluorescein DHPE is a phospholipid that is labeled on the head group with the bright, green-fluorescent fluorescein dye. Because of fluorescein's photolability, fluorescein DHPE is a useful reagent for measuring lateral diffusion in membranes using fluorescence photobleaching recovery methods. Fluorescein DHPE has also been used in conjunction with polyclonal anti-fluorescein dye antibody to prepare colloidal gold probes for SPT diffusion measurements in supported phospholipid bilayers and in keratocyte plasma membranes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	84.563 µL	422.815 µL	845.63 µL	4.228 mL	8.456 mL
5 mM	16.913 µL	84.563 µL	169.126 µL	845.63 µL	1.691 mL
10 mM	8.456 µL	42.282 µL	84.563 µL	422.815 µL	845.63 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein biotin	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein dicaprylate [Fluorescein dioctanoate]	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
TRITC-DHPE	544	570	100000	-	0.27	0.34
Fluorescein aldehyde [5-FAM aldehyde]	493	517	83000	-	0.32	0.178
Fluorescein Tyramide	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
OG488 DHPE	498	526	76000	-	0.31	0.12

Citations

View all 5 citations: Citation Explorer

Highly Robust Multilamellar Lipid Vesicles Generated through Intervesicular Self-assembly Mediated by Hydrolyzed Collagen Peptides
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)

Activation of Nedd4L Ubiquitin Ligase by FCHO2-generated Membrane Curvature
Authors: Sakamoto, Yasuhisa and Uezu, Akiyoshi and Kikuchi, Koji and Suetsugu, Shiro and Nakanishi, Hiroyuki
Journal: bioRxiv (2022)

DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates
Authors: Glazier, Roxanne and Brockman, Joshua M and Bartle, Emily and Mattheyses, Alexa L and Destaing, Olivier and Salaita, Khalid
Journal: Nature Communications (2019): 1--13

Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2018): 74--85

References

View all 27 references: Citation Explorer

Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767

Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861

Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134

Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005

Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221

Page updated on December 17, 2024

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Physical properties

Molecular weight	1182.55
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200