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Fluorescein DHPE
Fluorescein DHPE is a phospholipid that is labeled on the head group with the bright, green-fluorescent fluorescein dye. Because of fluorescein's photolability, fluorescein DHPE is a useful reagent for measuring lateral diffusion in membranes using fluorescence photobleaching recovery methods. Fluorescein DHPE has also been used in conjunction with polyclonal anti-fluorescein dye antibody to prepare colloidal gold probes for SPT diffusion measurements in supported phospholipid bilayers and in keratocyte plasma membranes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 84.563 µL | 422.815 µL | 845.63 µL | 4.228 mL | 8.456 mL |
| 5 mM | 16.913 µL | 84.563 µL | 169.126 µL | 845.63 µL | 1.691 mL |
| 10 mM | 8.456 µL | 42.282 µL | 84.563 µL | 422.815 µL | 845.63 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Fluorescein biotin | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| Fluorescein dicaprylate [Fluorescein dioctanoate] | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| TRITC-DHPE | 544 | 570 | 100000 | - | 0.27 | 0.34 |
| Fluorescein aldehyde [5-FAM aldehyde] | 493 | 517 | 83000 | - | 0.32 | 0.178 |
| Fluorescein Tyramide | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| OG488 DHPE | 498 | 526 | 76000 | - | 0.31 | 0.12 |
Citations
View all 5 citations: Citation Explorer
Highly Robust Multilamellar Lipid Vesicles Generated through Intervesicular Self-assembly Mediated by Hydrolyzed Collagen Peptides
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)
Authors: Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung
Journal: (2023)
Activation of Nedd4L Ubiquitin Ligase by FCHO2-generated Membrane Curvature
Authors: Sakamoto, Yasuhisa and Uezu, Akiyoshi and Kikuchi, Koji and Suetsugu, Shiro and Nakanishi, Hiroyuki
Journal: bioRxiv (2022)
Authors: Sakamoto, Yasuhisa and Uezu, Akiyoshi and Kikuchi, Koji and Suetsugu, Shiro and Nakanishi, Hiroyuki
Journal: bioRxiv (2022)
DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates
Authors: Glazier, Roxanne and Brockman, Joshua M and Bartle, Emily and Mattheyses, Alexa L and Destaing, Olivier and Salaita, Khalid
Journal: Nature Communications (2019): 1--13
Authors: Glazier, Roxanne and Brockman, Joshua M and Bartle, Emily and Mattheyses, Alexa L and Destaing, Olivier and Salaita, Khalid
Journal: Nature Communications (2019): 1--13
Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2018): 74--85
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2018): 74--85
Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)
References
View all 27 references: Citation Explorer
Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
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