Fluorescein aldehyde [5-FAM aldehyde]

Fluorescein aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Fluorescein is the most widely used green fluorescent dye. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated fluorescein dye can be easily detected by a common fluorescence instrument under FITC channel.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein biotin	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein dicaprylate [Fluorescein dioctanoate]	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein DHPE	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000	-	0.32	0.178
Cy3 aldehyde	555	569	150000¹	0.15¹	0.07	0.073
Cy5 aldehyde	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	499	520	71000	0.92¹	0.30	0.11
Fluorescein Tyramide	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

References

View all 50 references: Citation Explorer

Preparation and optical parameter characterization of two aldehyde derivative thin films for photonic applications by drop casting method.
Authors: Melavanki, Raveendra and Vaijayanthimala, S and Yallur, Basappa Chanabasappa and Shelar, Vikas M and Singh, Diksha and Sadasivuni, Kishor Kumar and Patil, N R
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912

Establishing sample-preparation protocols for X-ray phase-contrast CT of rodent spinal cords: Aldehyde fixations and osmium impregnation.
Authors: Barbone, Giacomo E and Bravin, Alberto and Mittone, Alberto and Kraiger, Markus J and Hrabě de Angelis, Martin and Bossi, Mario and Ballarini, Elisa and Rodriguez-Menendez, Virginia and Ceresa, Cecilia and Cavaletti, Guido and Coan, Paola
Journal: Journal of neuroscience methods (2020): 108744

Characterization of ascites-derived aldehyde dehydrogenase-positive ovarian cancer stem cells isolated from Leghorn chickens.
Authors: Tiwari, Anupama and Hadley, Jill A and Ramachandran, Ramesh
Journal: Poultry science (2020): 2203-2214

Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling.
Authors: Mortensen, Michael R and Skovsgaard, Mikkel B and Märcher, Anders and Andersen, Veronica L and Palmfeldt, Johan and Nielsen, Thorbjørn B and Tørring, Thomas and Laursen, Nick S and Andersen, Kasper R and Kjems, Jørgen and Gothelf, Kurt V
Journal: Bioconjugate chemistry (2020): 1295-1300

2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170

Page updated on November 20, 2024

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Unit size	5 mg 1 mg
Catalog Number	90009001
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Physical properties

Molecular weight	550.52
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501